Beclin 1 Antibody (22F4) - BSA Free

Novus Biologicals | Catalog # NBP3-26478

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 22F4 expressed in HEK293

Format

BSA Free
View all Beclin 1 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human Beclin 1 [UniProt Q14457]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit Beclin 1 Antibody (22F4) - BSA Free (NBP3-26478) is a recombinant monoclonal antibody validated for use in ELISA and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Beclin 1 Antibody (22F4) - BSA Free

Beclin 1 Antibody (22F4)

Flow Cytometry: Beclin 1 Antibody (22F4) [NBP3-26478] -

Flow Cytometry: Beclin 1 Antibody (22F4) [NBP3-26478] - Overlay Peak curve showing RAJI cells stained with Beclin 1 Antibody (22F4) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

Applications for Beclin 1 Antibody (22F4) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:50-1:200

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: Beclin 1

Beclin 1 was the first mammalian gene identified to mediate autophagy. Autophagy, a process of bulk protein degradation via an autophagosomic-lysosomal pathway, is critical for for proper differentiation, cell maintenence during nutrient deprivation, and normal growth control, but is often defective in tumor cells. Together with its binding partner, PI3K, Beclin-1 forms a clomplex that is required to initiate the formation of the autophagasome.

Beclin-1 encodes an evolutionarily conserved 60kDa coiled-coil protein that is expressed in human muscle, epithelial cells and neurons. In gene transfer studies, beclin 1 promotes nutrient deprivation-induced autophagy, inhibits mammary tumorigenesis, and inhibits viral replication.

Expression of the Beclin1 protein is frequently decreased in malignant breast epithelial cells. Based upon these observations, it is speculated that beclin-1 may work through induction of autophagy to negatively regulate tumorigenesis and to control viral infections. Beclin 1 may also play a role in other biological processes in which autophagy is important such as cell differentiation and nutritional stress responses.

Long Name

Beclin1, Autophagy-related

Alternate Names

ATG6, BECN1, GT197, VPS30

Gene Symbol

BECN1

Additional Beclin 1 Products

Product Documents for Beclin 1 Antibody (22F4) - BSA Free

Certificate of Analysis

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Product Specific Notices for Beclin 1 Antibody (22F4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Autophagy

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Protocols

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FAQs

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Associated Pathways

Akt Signaling Pathway Akt Signaling Pathway Thumbnail