beta-III Tubulin Antibody (CL5813)

Novus Biologicals | Catalog # NBP3-52481

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # CL5813
View all beta-III Tubulin Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a synthetic peptide derived from Q13509, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for beta-III Tubulin Antibody (CL5813)

Immunocytochemistry/ Immunofluorescence: beta-III Tubulin Antibody (CL5813) [NBP3-52481]

Immunocytochemistry/ Immunofluorescence: beta-III Tubulin Antibody (CL5813) [NBP3-52481]

Staining of A549 cells showing specific staining on microtubules in green. Microtubule and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5813) [NBP3-52481]

Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5813) [NBP3-52481]

Analysis in human cerebral cortex and skeletal muscle tissues. Corresponding RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5813) [NBP3-52481]

Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5813) [NBP3-52481]

Staining of human cerebellum shows strong positivity in neurons and neuropil.
Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5813) [NBP3-52481]

Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5813) [NBP3-52481]

Staining of human cerebral cortex shows strong positivity in neurons and neuropil.
Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5813) [NBP3-52481]

Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5813) [NBP3-52481]

Staining of human prostate shows strong positivity in peripheral nerves.
Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5813) [NBP3-52481]

Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5813) [NBP3-52481]

Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5813) [NBP3-52481]

Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5813) [NBP3-52481]

Staining of mouse embryo E11 shows strong positivity in the developing central and peripheral nervous system.
Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5813) [NBP3-52481]

Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5813) [NBP3-52481]

Staining of rat brain shows strong cytoplasmic positivity in neurons.
Immunocytochemistry/ Immunofluorescence: beta-III Tubulin Antibody (CL5813) [NBP3-52481]

Immunocytochemistry/ Immunofluorescence: beta-III Tubulin Antibody (CL5813) [NBP3-52481]

Staining of SH-SY5Y cells showing specific staining on microtubules in green. Nuclear probe is visualized in blue.

Applications for beta-III Tubulin Antibody (CL5813)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:50000 - 1:100000

Immunohistochemistry-Paraffin

1:50000 - 1:100000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Preservative

0.02% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: beta-III Tubulin

Beta-III Tubulin is abundant in the central and peripheral nervous systems where it is prominently expressed during fetal and postnatal development. Beta III Tubulin is often referred to as TUJ1 because Tuj-1 is the clone name of the original monoclonal antibody. The Tuj1 clone is such a popular neuronal marker that Tuj-1 is now used as a synonym for beta III tubulin. The distribution of Tubulin Beta 3 is neuron-associated, exhibiting distinct temporospatial gradients according to the regional neuroepithelia of origin. However, transient expression of the Tubulin Beta 3 protein is also present in the subventricular zones of the CNS comprising putative neuronal- and/or glial precursor cells, as well as in Kulchitsky neuroendocrine cells of the fetal respiratory epithelium. This temporally restricted, potentially non-neuronal expression may have implications in the identification of presumptive neurons derived from embryonic stem cells. Mutation on beta-tubulin has been linked to anti-cancer drug resistance in lung cancers.

Alternate Names

beta-Tubulin III, Beta3-tubulin, betaIII Tubulin, Class III beta-tubulin, TUBB3, Tubulin Beta 3, Tubulin beta-4, Tubulin beta-III

Gene Symbol

TUBB3

Additional beta-III Tubulin Products

Product Documents for beta-III Tubulin Antibody (CL5813)

Certificate of Analysis

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Product Specific Notices for beta-III Tubulin Antibody (CL5813)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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