BLAME, also known as Signaling Lymphocyte Activation Molecule Family Member 8 (SLAMF8), is an Ig superfamily protein that is a member of the SLAM/CD150/CD2 family of receptors. It is expressed on macrophages and dendritic cells. BLAME modulates signaling through the B cell receptor and may also function during B lineage commitment.
BLAME/SLAMF8 Antibody
Novus Biologicals | Catalog # NBP2-26110
![Western Blot: BLAME/SLAMF8 Antibody [NBP2-26110]](https://beta-resources.rndsystems.com/images/products/BLAME-SLAMF8-Antibody-Western-Blot-NBP2-26110-img0001.jpg "Western Blot: BLAME/SLAMF8 Antibody [NBP2-26110]")
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Peptide with sequence C-TLYHSRFLGRAQ corresponding to internal region according to NP_064510.1.
Reactivity Notes
Expected from sequence similarity: Mouse, Rat, Bovine
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
Novus Biologicals Goat BLAME/SLAMF8 Antibody (NBP2-26110) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-BLAME/SLAMF8 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for BLAME/SLAMF8 Antibody
Western Blot: BLAME/SLAMF8 Antibody [NBP2-26110]
Western Blot: BLAME/SLAMF8 Antibody [NBP2-26110] - HEK293 lysate (10 ug protein in RIPA buffer) overexpressing Human SLAMF8 with C-terminal MYC tag probed with NBP2-26110 (1 ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with NBP2-26110 (1 ug/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.![Immunohistochemistry-Paraffin: BLAME/SLAMF8 Antibody [NBP2-26110]](https://beta-resources.rndsystems.com/images/products/BLAME-SLAMF8-Antibody-Immunohistochemistry-Paraffin-NBP2-26110-img0002.jpg "Immunohistochemistry-Paraffin: BLAME/SLAMF8 Antibody [NBP2-26110]")
Immunohistochemistry-Paraffin: BLAME/SLAMF8 Antibody [NBP2-26110]
Immunohistochemistry-Paraffin: BLAME/SLAMF8 Antibody [NBP2-26110] - 5ug/ml staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Applications for BLAME/SLAMF8 Antibody
Application
Recommended Usage
Immunohistochemistry
0.5-1ug/ml
Peptide ELISA
Detection limit 1:8000
Western Blot
1:100 - 1:2000
Application Notes
Western blot: In transfected HEK293 transiently expressing Human SLAMF8 (myc and DYKDDDDK tagged), a band of approx. 45kDa is observed. No bands are observed in mock-transfected HEK293 and the same band is observed using anti-myc antibody.
IHC: Paraffin embedded Human Small Intestine.
IHC: Paraffin embedded Human Small Intestine.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: BLAME/SLAMF8
Long Name
SLAM Family Member 8
Alternate Names
CD353, SBBI42, SLAMF8
Gene Symbol
SLAMF8
Additional BLAME/SLAMF8 Products
Product Documents for BLAME/SLAMF8 Antibody
Product Specific Notices for BLAME/SLAMF8 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for BLAME/SLAMF8 Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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