BOB1 Antibody (5J7A6)

Name: BOB1 Antibody (5J7A6)
Brand: Novus Biologicals
SKU: NBP3-57398
Price: 399 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-57398

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5J7A6

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Product Specifications

Immunogen

Synthetic peptide. This information is considered to be commercially sensitive. (Swissprot# Q16633)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is highly specific and optimized for use in Immunohistochemistry.

Normal Tissues Tested
Fallopian tube, Pancreas, Lung, Kidney, Placenta, Stomach, Liver, Thyroid, Appendix, Testis, Prostate, Tonsil, Cervix, esophagus, Breast, Colon, Brain

Cancer Tissues Tested
Lung adenocarcinoma, Lung squamous carcinoma, Hepatocellular Carcinoma, Hepatocholangio carcinoma, Thyroid Cancer, Prostate cancer, cervical squamous cell carcinoma, Breast Cancer, Colon Cancer, B-cell lymphoma, follicular lymphoma

Positive Controls
Tonsil, spleen, B-cell lymphoma, follicular lymphoma

Negative Controls
Epithelial cells. tonsil (epithelial cells), Fallopian tube, Pancreas, Kidney, Placenta, Stomach, Liver, Thyroid, Brain

Applications for BOB1 Antibody (5J7A6)

Application

Recommended Usage

ELISA

1 ug/mL

Immunocytochemistry/ Immunofluorescence

1:200 - 1:400

Immunohistochemistry-Paraffin

1:400 - 1:1600

Western Blot

1:12000 - 1:72000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, (pH 7.3)

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: BOB1

BOB1 is a novel 35kDa protein designated Bob1 (B cell Oct binding protein 1) is a lymphocyte specific transcription co activator protein. B cell specific transcription is mediated by octamer motifs found in immunoglobulin heavy and light chain gene promoters and in some immunoglobulin enhancers. These sites are bound by the B cell specific Oct1 and Oct2 transcription factors, and the B cell restricted co activator BOB.1/OBF.1. This protein interacts only with the Oct1/2 proteins through sub domains in the POU domain of the Oct1/2 proteins, enhancing their transcriptional efficacy. Although having no intrinsic capacity for DNA binding, Bob1 associates tightly with the octomer motif in the presence of Oct1 and Oct2. Bob1 is expressed at highest levels in spleen and peripheral blood leukocytes.

Alternate Names

B-cell-specific coactivator OBF-1, BOB-1, OBF-1, OBF1BOB1, OCAB, OCA-B, OCT-binding factor 1, POU class 2 associating factor 1, POU domain class 2, associating factor 1, POU domain class 2-associating factor 1, POU domain, class 2, associating factor 1

Gene Symbol

POU2AF1

Additional BOB1 Products

Product Documents for BOB1 Antibody (5J7A6)

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Certificate of Analysis

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Product Specific Notices for BOB1 Antibody (5J7A6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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