BRCA1 Antibody (6B4) - Azide and BSA Free

Novus Biologicals | Catalog # NB100-404

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown, Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Knockdown Validated

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence, Chemotaxis, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 6B4

Format

Azide and BSA Free
View all BRCA1 Primary Antibodies »

Product Specifications

Immunogen

BRCA1 protein fragment expressed in E. coli corresponding to amino acids 341-748.

Localization

Nuclear

Specificity

This recognizes BRCA1, a 220-kDa nuclear phosphoprotein, and does not recognize the exon 11 splice variant. Mutations in this tumor suppressor gene greatly increase the risk of breast cancer.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

220 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Knockout (KO) Validated Mouse BRCA1 Antibody (6B4) - Azide and BSA Free (NB100-404) is a monoclonal antibody validated for use in IHC, WB, ICC/IF, IP and ChIP. Anti-BRCA1 Antibody: Cited in 10 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for BRCA1 Antibody (6B4) - Azide and BSA Free

Western Blot: BRCA1 Antibody (6B4) [NB100-404]

Western Blot: BRCA1 Antibody (6B4) [NB100-404]

Western Blot: BRCA1 Antibody (6B4) [NB100-404] - Whole cell extracts (30 and 50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody at a dilution of 1:500.
Immunocytochemistry/ Immunofluorescence: BRCA1 Antibody (6B4) [NB100-404]

Immunocytochemistry/ Immunofluorescence: BRCA1 Antibody (6B4) [NB100-404]

Immunocytochemistry/Immunofluorescence: BRCA1 Antibody (6B4) [NB100-404] - Staining of BRCA1 nuclear foci induced by ionizing radiation. IR-treated (2 hr /4 gray IR) U2OS cells were pre-extracted with CSK buffer on ice for 4 min before fixation with 4% PFA in room temperature, and then subjected to immunostaining. DAPI was used to counterstain nucleus. 6B4 was used. Secondary antibody (Alexa Fluor-488) used for detection of primary antibody (BRCA1 antibody 6B4)
Knockdown Validated: BRCA1 Antibody (6B4) [NB100-404]

Western Blot: BRCA1 Antibody (6B4) [NB100-404]

Western Blot: BRCA1 Antibody (6B4) [NB100-404] - Non-transfected (-) and transfected (+) 293T whole cell extracts (60 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [6B4] - ChIP grade diluted at 1:500.
Knockdown Validated: BRCA1 Antibody (6B4) [NB100-404]

Western Blot: BRCA1 Antibody (6B4) [NB100-404]

Western Blot: BRCA1 Antibody (6B4) [NB100-404] - NB100-404 was used at 1:1000 dilution for Western blot assay of lysates from cells transfected with control or BRCA1-specific siRNA. Lysates were prepared at the indicated times following transfection. RAD50 antibody (13B3) (NB100-147) was used as a loading control.
Immunoprecipitation: BRCA1 Antibody (6B4) [NB100-404]

Immunoprecipitation: BRCA1 Antibody (6B4) [NB100-404]

Immunoprecipitation: BRCA1 Antibody (6B4) [NB100-404] - 6B4 alone (4 microgram), 17F8 alone (4 microgram), 6B4 plus 17F8 (2 microgram each), and mouse control normal IgG were used in an immunoprecipitation assay with MCF7 cell extract. Immunoprecipitated BRCA1 was detected in WB using BRCA1 antibody 6B4 at 1:1000 dilution. HeLa whole cell extract (20 microgram) was used as input in the Western blot assay.
Knockdown Validated: BRCA1 Antibody (6B4) [NB100-404]

Western Blot: BRCA1 Antibody (6B4) [NB100-404]

BRCA1-Antibody-6B4-Knockdown-Validated-NB100-404-img0014.jpg
Knockout Validated: BRCA1 Antibody (6B4) [NB100-404]

Western Blot: BRCA1 Antibody (6B4) [NB100-404] -

Western Blot: BRCA1 Antibody (6B4) [NB100-404] - Wild-type (WT) and BRCA1 knockout (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody, diluted at 1:500. A HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
BRCA1 Antibody (6B4)

Western Blot: BRCA1 Antibody (6B4) [NB100-404] -

Western Blot: BRCA1 Antibody (6B4) [NB100-404] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [6B4] - ChIP grade (NB100-404) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.
BRCA1 Antibody (6B4) - Azide and BSA Free

Chromatin Immunoprecipitation (ChIP): BRCA1 Antibody (6B4) [NB100-404] -

BRCA1 antibody 6B4 (NB100-404) and BRCA1 antibody 17F8 was validated by a Q-PCR assay.
BRCA1 Antibody (6B4) - Azide and BSA Free

Immunohistochemistry: BRCA1 Antibody (6B4) [NB100-404] -

Applications for BRCA1 Antibody (6B4) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Paraffin

1:10 - 1:500

Western Blot

1:500-1:3000
Application Notes
IHC reactivity reported in (PMID: 10910365).

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS, 20% Glycerol

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BRCA1

BRCA1 (breast and ovarian cancer susceptibility protein 1) is a nuclear phosphoprotein that plays a role in maintaining genomic stability and acts as a tumor suppressor. It combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as BASC (for BRCA1 associated genome surveillance complex). BRCA1 associates with RNA polymerase II, and through the C terminal domain, also interacts with histone deacetylase complex. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in the BRCA1 gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants have been described for this gene but only some have had their full length natures identified.

Long Name

Breast Cancer 1

Alternate Names

BRCAI, breast and ovarian cancer susceptibility protein 1, breast and ovarian cancer sususceptibility protein, breast cancer 1, early onset, breast cancer type 1 susceptibility protein, EC 6.3.2, EC 6.3.2.-, IRIS, PNCA4, PSCP, subunit 1

Entrez Gene IDs

672 (Human)

Gene Symbol

BRCA1

Additional BRCA1 Products

Product Documents for BRCA1 Antibody (6B4) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for BRCA1 Antibody (6B4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for BRCA1 Antibody (6B4) - Azide and BSA Free

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Protocols

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