Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # RAY

Format

Azide and BSA Free
View all BRCA1 Primary Antibodies »

Product Specifications

Immunogen

Human BRCA1 corresponding to residues 1314-1864 [UniProt# P38398]

Epitope

The epitope recognized is found between amino acids 1314-1600.

Localization

nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

230 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse BRCA1 Antibody (RAY) - Azide and BSA Free (NB100-598) is a monoclonal antibody validated for use in WB, Flow, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for BRCA1 Antibody (RAY) - Azide and BSA Free

Western Blot: BRCA1 Antibody (RAY)Azide and BSA Free [NBP2-80600]

Western Blot: BRCA1 Antibody (RAY)Azide and BSA Free [NBP2-80600]

Western Blot: BRCA1 Antibody (RAY) - Azide and BSA Free [NBP2-80600] - Analysis of BRCA1 on MCF7 lysate. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: BRCA1 Antibody (RAY) - Azide and BSA Free [NBP2-80600]

Immunocytochemistry/ Immunofluorescence: BRCA1 Antibody (RAY) - Azide and BSA Free [NBP2-80600]

Immunocytochemistry/Immunofluorescence: BRCA1 Antibody (RAY) - Azide and BSA Free [NBP2-80600] - BRCA (RAY) antibody was tested in MCF-7 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). Image from the standard format of this antibody.
Flow Cytometry: BRCA1 Antibody (RAY) - Azide and BSA Free [NBP2-80600]

Flow Cytometry: BRCA1 Antibody (RAY) - Azide and BSA Free [NBP2-80600]

Flow Cytometry: BRCA1 Antibody (RAY) - Azide and BSA Free [NBP2-80600] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using BRCA1 antibody (dark blue) and isotype control (orange). An antibody concentration of 1 ug/1x10^6 cells was used. Image from the standard format of this antibody.

Applications for BRCA1 Antibody (RAY) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1 ug per million cells

Immunocytochemistry/ Immunofluorescence

1:100

Immunoprecipitation

1:10-1:500

Western Blot

2-4 ug/ml
Application Notes
This BRCA1 antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence and Immunoprecipitation. In Western blot a band was observed ~220-240 kDa. This antibody is CyTOF ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: BRCA1

BRCA1 (breast cancer early-onset 1) is a tumour-suppressor gene which was originally discovered on the basis of its linkage to early-onset breast or ovarian cancer in women. BRCA1 gene encodes a protein of 1863 amino acids with three distinct regions of protein interaction: RING domain, RAD51-interaction domain and BRCT (BRCA1 C-terminus) domain. The N-terminal RING finger domain binds to several proteins, including formation of heterodimers with BARD1 (BRCA1-associated RING domain protein). Both BRCA1 and BARD1 contain the RING finger domain which is important for tumour-suppression functions. The RAD51-interaction domain is enclosed by exon 11 and is involved in DNA-DSBs repair. It also contains multiple protein-binding sites, in addition to those for RAD51/RAD50 complex. Loss of RAD51 binding increase the risk of cancer as there could be an increment in the amount of damaged DNA. The C-terminus of BRCA1 contains an acidic domain that functions as a transcriptional activation domain and this domain contains a tandem repeat of approximately 95 amino acids called BRCT. This domain is also found in proteins such as BARD1 which is involved in cell-cycle control and DNA repair. BRCA1 has been implicated in diverse cancer-related activities, including roles in cell-cycle progression, DNA repair, DNA damage-responsive cell-cycle checkpoints, transcription regulation, ubiquitination, chromosome remodelling and apoptosis. Defects in BRCA1 are a cause of susceptibility to breast cancer, familial breast-ovarian cancer type 1 (BROVCA1), ovarian cancer, and pancreatic cancer type 4 (PNCA4).

Long Name

Breast Cancer 1

Alternate Names

BRCAI, breast and ovarian cancer susceptibility protein 1, breast and ovarian cancer sususceptibility protein, breast cancer 1, early onset, breast cancer type 1 susceptibility protein, EC 6.3.2, EC 6.3.2.-, IRIS, PNCA4, PSCP, subunit 1

Gene Symbol

BRCA1

Additional BRCA1 Products

Product Documents for BRCA1 Antibody (RAY) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for BRCA1 Antibody (RAY) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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