BYSL Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89500

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all BYSL Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QQQEELEAEHGTGDKPAAPRERTTRLGPRMPQDGSDDEDEEWPTLEKAATMTAAGHHAEVVVDPEDERAIEMFMNKNPPAR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BYSL Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: BYSL Antibody [NBP1-89500]

Immunocytochemistry/ Immunofluorescence: BYSL Antibody [NBP1-89500]

Immunocytochemistry/Immunofluorescence: BYSL Antibody [NBP1-89500] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus, nucleoli & vesicles.
Immunohistochemistry-Paraffin: BYSL Antibody [NBP1-89500]

Immunohistochemistry-Paraffin: BYSL Antibody [NBP1-89500]

Immunohistochemistry-Paraffin: BYSL Antibody [NBP1-89500] - Staining of human gastrointestinal, lymphoid tissues, placenta and testis using Anti-BYSL antibody NBP1-89500 (A) shows similar protein distribution across tissues to independent antibody NBP1-89501 (B).
Immunohistochemistry-Paraffin: BYSL Antibody [NBP1-89500]

Immunohistochemistry-Paraffin: BYSL Antibody [NBP1-89500]

Immunohistochemistry-Paraffin: BYSL Antibody [NBP1-89500] - Staining of human testis shows moderate positivity in nucleoli in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: BYSL Antibody [NBP1-89500]

Immunohistochemistry-Paraffin: BYSL Antibody [NBP1-89500]

Immunohistochemistry-Paraffin: BYSL Antibody [NBP1-89500] - Staining of human placenta.
Immunohistochemistry-Paraffin: BYSL Antibody [NBP1-89500]

Immunohistochemistry-Paraffin: BYSL Antibody [NBP1-89500]

Immunohistochemistry-Paraffin: BYSL Antibody [NBP1-89500] - Staining of human placenta shows strong positivity in nucleoli in trophoblastic cells.
Immunohistochemistry: BYSL Antibody [NBP1-89500]

Immunohistochemistry: BYSL Antibody [NBP1-89500]

Immunohistochemistry: BYSL Antibody [NBP1-89500] - Staining of human lymph node shows strong positivity in nucleoli in lymphoid cells.
Immunohistochemistry-Paraffin: BYSL Antibody [NBP1-89500]

Immunohistochemistry-Paraffin: BYSL Antibody [NBP1-89500]

Immunohistochemistry-Paraffin: BYSL Antibody [NBP1-89500] - Staining of human rectum shows strong positivity in nucleoli in glandular cells.
Western Blot: BYSL Antibody [NBP1-89500]

Western Blot: BYSL Antibody [NBP1-89500]

Western Blot: BYSL Antibody [NBP1-89500] - Analysis using Anti-BYSL antibody NBP1-89500 (A) shows similar pattern to independent antibody NBP1-89501 (B).

Applications for BYSL Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BYSL

Bystin is expressed as a 2-kb major transcript and a 3.6-kb minor transcript in SNG-M cells and in human trophoblastic teratocarcinoma HT-H cells. Protein binding assays determined that bystin binds directly to trophinin and tastin, and that binding is enhanced when cytokeratins 8 and 18 are present. Immunocytochemistry of HT-H cells showed that bystin colocalizes with trophinin, tastin, and the cytokeratins, suggesting that these molecules form a complex in trophectoderm cells at the time of implantation. Using immunohistochemistry it was determined that trophinin and bystin are found in the placenta from the sixth week of pregnancy. Both proteins were localized in the cytoplasm of the syncytiotrophoblast in the chorionic villi and in endometrial decidual cells at the uteroplacental interface. After week 10, the levels of trophinin, tastin, and bystin decreased and then disappeared from placental villi.

Alternate Names

by the ribosomal protein s6 gene, drosophila, homolog-like, BYSTIN, bystin-like

Gene Symbol

BYSL

Additional BYSL Products

Product Documents for BYSL Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for BYSL Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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