CAD Antibody (SR1408) - BSA Free

Novus Biologicals | Catalog # NBP3-22169

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR1408 expressed in HEK293

Format

BSA Free
View all CAD Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human CAD (Uniprot #: P27708)

Specificity

Detects endogenous levels of total CAD

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

260 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CAD Antibody (SR1408) - BSA Free

Western Blot: CAD Antibody (SR1408) [NBP3-22169] -

Western Blot: CAD Antibody (SR1408) [NBP3-22169] - Western blot analysis using NBP3-22169 in HeLa cell lysate.

Applications for CAD Antibody (SR1408) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:80

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunoprecipitation

1:30

Western Blot

1:500-1:1000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CAD

Carbamoyl phosphate synthetase-aspartate carbamoyltransferase-dihydroorotase (CAD) is a multifunctional protein that initiates and regulates mammalian de novo pyrimidine biosynthesis (1). This trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis is the rate-limiting step in the de novo pyrimidine synthetic pathway (2). Although most of the CAD protein in the cell is cytosolic, phosphorylation at threonine 456 localizes the protein to the nucleus (3). While MAPK and EGF phosphorylate CAD at threonine 456, MAPK and c-myc have been found to induce over-expression of CAD (4-5).

Alternate Names

CAD protein, CAD trifunctional protein, carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, anddihydroorotase, EC 2.1.3.2, EC 3.5.2.3, EC 6.3.5, EC 6.3.5.5, multifunctional protein CAD

Gene Symbol

CAD

Additional CAD Products

Product Documents for CAD Antibody (SR1408) - BSA Free

Certificate of Analysis

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Product Specific Notices for CAD Antibody (SR1408) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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