Cadherin-17 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-88237

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Cadherin-17 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GTKVGNVTAKDPEGLDISYSLRGDTRGWLKIDHVTGEIFSVAPLDREAGSPYRVQVVATEVGGSSLSSVSEFHLILMDVNDNPPRLAKDYT

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%), Rat (80%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cadherin-17 Antibody - BSA Free

Western Blot: Cadherin-17 Antibody [NBP1-88237]

Western Blot: Cadherin-17 Antibody [NBP1-88237]

Western Blot: Cadherin-17 Antibody [NBP1-88237] - Analysis in human cell lines Caco-2 and SK-MEL-30 using anti-CDH17 antibody. Corresponding CDH17 RNA-seq data are presented for the same cell lines. Loading control: anti-HDAC1.
Cadherin-17 Antibody Immunohistochemistry-Paraffin: Cadherin-17 Antibody Antibody [NBP1-88237]

Immunohistochemistry-Paraffin: Cadherin-17 Antibody Antibody [NBP1-88237]

Staining of human colon, duodenum, kidney and liver using NBP1-88237 (A) shows similar protein distribution across tissues to independent antibody NBP1-88239 (B).
Immunohistochemistry-Paraffin: Cadherin-17 Antibody [NBP1-88237]

Immunohistochemistry-Paraffin: Cadherin-17 Antibody [NBP1-88237]

Immunohistochemistry-Paraffin: Cadherin-17 Antibody [NBP1-88237] - Analysis in human duodenum and liver tissues using NBP1-88237 antibody. Corresponding Cadherin-17 RNA-seq data are presented for the same tissues.
Cadherin-17 Antibody - BSA Free Immunohistochemistry-Paraffin: Cadherin-17 Antibody - BSA Free [NBP1-88237]

Immunohistochemistry-Paraffin: Cadherin-17 Antibody - BSA Free [NBP1-88237]

Staining of human duodenum shows strong membranous positivity in glandular cells.
Cadherin-17 Antibody - BSA Free Immunohistochemistry-Paraffin: Cadherin-17 Antibody - BSA Free [NBP1-88237]

Immunohistochemistry-Paraffin: Cadherin-17 Antibody - BSA Free [NBP1-88237]

Staining of human liver shows no positivity in hepatocytes as expected.
Cadherin-17 Antibody - BSA Free Immunohistochemistry-Paraffin: Cadherin-17 Antibody - BSA Free [NBP1-88237]

Immunohistochemistry-Paraffin: Cadherin-17 Antibody - BSA Free [NBP1-88237]

Staining of human colon shows strong membranous positivity in glandular cells.
Cadherin-17 Antibody - BSA Free Immunohistochemistry-Paraffin: Cadherin-17 Antibody - BSA Free [NBP1-88237]

Immunohistochemistry-Paraffin: Cadherin-17 Antibody - BSA Free [NBP1-88237]

Staining of human kidney shows no positivity in cells in tubules as expected.

Applications for Cadherin-17 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cadherin-17

LI Cadherin is a member of the cadherin superfamily, genes encoding calcium-dependent, membrane-associated glycoproteins. The encoded protein is cadherin-like, consisting of an extracellular region, containing 7 cadherin domains, and a transmembrane region but lacking the conserved cytoplasmic domain. The protein is a component of the gastrointestinal tract and pancreatic ducts, acting as an intestinal proton-dependent peptide transporter in the first step in oral absorption of many medically important peptide-based drugs. The protein may also play a role in the morphological organization of liver and intestine.

Alternate Names

Cadherin17, CDH17

Gene Symbol

CDH17

Additional Cadherin-17 Products

Product Documents for Cadherin-17 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Cadherin-17 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Adhesion Molecules
Cadherin Superfamily

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Protocols

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