Calpain S1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-90329

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Calpain S1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: THYSNIEANESEEVRQFRRLFAQLAGDDMEVSATELMNILNKVVTRHPDLKTDGFGIDTCRSMVAVMDSDTTGKLGFEEFKYLWNNIKRWQAIYKQFDTDRSGTICSSELPG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Calpain S1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Calpain S1 Antibody [NBP1-90329]

Immunocytochemistry/ Immunofluorescence: Calpain S1 Antibody [NBP1-90329]

Immunocytochemistry/Immunofluorescence: Calpain S1 Antibody [NBP1-90329] - Staining of human cell line A-431 shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Calpain S1 Antibody [NBP1-90329]

Immunohistochemistry-Paraffin: Calpain S1 Antibody [NBP1-90329]

Immunohistochemistry-Paraffin: Calpain S1 Antibody [NBP1-90329] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.
Calpain S1 Antibody - BSA Free Western Blot: Calpain S1 Antibody - BSA Free [NBP1-90329]

Western Blot: Calpain S1 Antibody - BSA Free [NBP1-90329]

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Calpain S1 Antibody - BSA Free Western Blot: Calpain S1 Antibody - BSA Free [NBP1-90329]

Western Blot: Calpain S1 Antibody - BSA Free [NBP1-90329]

Analysis in human cell lines A-549 and HEK293 using Anti-CAPNS1 antibody. Corresponding CAPNS1 RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.

Applications for Calpain S1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Calpain S1

This gene is a member of the calpain small subunit family. Calpains are calcium-dependent cysteine proteinases that are widely distributed in mammalian cells. Calpains operate as heterodimers, comprising a specific large catalytic subunit (calpain 1 subunit in Calpain I, and calpain 2 subunit in Calpain II), and a common small regulatory subunit encoded by this gene. This encoded protein is essential for the stability and function of both calpain heterodimers, whose proteolytic activities influence various cellular functions including apoptosis, proliferation, migration, adhesion, and autophagy. Calpains have been implicated in neurodegenerative processes, such as myotonic dystrophy. A pseudogene of this gene has been defined on chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]

Alternate Names

Calcium-activated neutral proteinase small subunit, Calcium-dependent protease small subunit 1, calcium-dependent protease, small subunit, calpain 4, small subunit (30K), Calpain regulatory subunit, calpain small subunit 1, calpain, small polypeptide, CALPAIN4, CANP, CANPS, CAPN4, CDPS, CSS1, epididymis secretory sperm binding protein

Gene Symbol

CAPNS1

Additional Calpain S1 Products

Product Documents for Calpain S1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Calpain S1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Calpain S1 Antibody - BSA Free

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Protocols

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