Canine IL-3 Antibody (662008)

R&D Systems | Catalog # MAB6658

R&D Systems

Key Product Details

Species Reactivity

Canine

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 662008
View all IL-3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant canine IL-3
Arg24-Pro143
Accession # Q9BDX4

Specificity

Detects canine IL-3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant IL-3 from human, mouse, or rat is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Canine IL-3 Antibody (662008)

IL-3 antibody in Canine PBMCs by Immunocytochemistry (ICC).

IL‑3 in Canine PBMCs.

IL-3 was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Canine IL-3 Monoclonal Antibody (Catalog # MAB6658) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Canine IL-3 Antibody (662008)

Application
Recommended Usage

Immunocytochemistry

20-30 µg/mL
Sample: Immersion fixed canine peripheral blood mononuclear cells

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-3

Interleukin-3 is a pleiotrophic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. IL-3 exerts its biological activities via a heterodimeric receptor composed of an IL-3 specific alpha chain and common beta chain that is shared with the IL-5 and GM-CSF high-affinity receptors. Receptors for IL-3 are present on bone marrow progenitors, and several mature myeloid cell types. Mature canine IL-3 shares <40% amino acid sequence identity with IL-3 of other mammals.

Long Name

Interleukin 3

Alternate Names

IL3, MCGF, MULTI-CSF

Entrez Gene IDs

3562 (Human); 16187 (Mouse); 24495 (Rat)

Gene Symbol

IL3

UniProt

Q9BDX4

Additional IL-3 Products

Product Documents for Canine IL-3 Antibody (662008)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Canine IL-3 Antibody (662008)

For research use only

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