Carboxypeptidase A1/CPA1 Antibody (CPA1/2712)

Novus Biologicals | Catalog # NBP2-79745

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Canine

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2c Kappa Clone # CPA1/2712
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Product Specifications

Immunogen

Recombinant full-length human Carboxypeptidase A1/CPA1 protein (Uniprot: P15085)

Localization

Secreted

Marker

Pancreatic Cancer Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2c Kappa

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-79880)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Carboxypeptidase A1/CPA1 Antibody (CPA1/2712)

Western Blot: Carboxypeptidase A1/CPA1 Antibody (CPA1/2712) [NBP2-79745]

Western Blot: Carboxypeptidase A1/CPA1 Antibody (CPA1/2712) [NBP2-79745]

Western Blot: Carboxypeptidase A1/CPA1 Antibody (CPA1/2712) [NBP2-79745] - Staining of human tonsil section. Image using the Alexa Fluor 488 format of this antibody.
Immunohistochemistry-Paraffin: Carboxypeptidase A1/CPA1 Antibody (CPA1/2712) [NBP2-79745]

Immunohistochemistry-Paraffin: Carboxypeptidase A1/CPA1 Antibody (CPA1/2712) [NBP2-79745]

Immunohistochemistry-Paraffin: Carboxypeptidase A1/CPA1 Antibody (CPA1/2712) [NBP2-79745] - Formalin-fixed, paraffin-embedded human Pancreas stained with Carboxypeptidase A1/CPA1 Antibody (CPA1/2712).
Immunohistochemistry-Paraffin: Carboxypeptidase A1/CPA1 Antibody (CPA1/2712) [NBP2-79745]

Immunohistochemistry-Paraffin: Carboxypeptidase A1/CPA1 Antibody (CPA1/2712) [NBP2-79745]

Immunohistochemistry-Paraffin: Carboxypeptidase A1/CPA1 Antibody (CPA1/2712) [NBP2-79745] - Formalin-fixed, paraffin-embedded human Pancreas stained with Carboxypeptidase A1/CPA1 Antibody (CPA1/2712).
Protein Array: Carboxypeptidase A1/CPA1 Antibody (CPA1/2712) [NBP2-79745]

Protein Array: Carboxypeptidase A1/CPA1 Antibody (CPA1/2712) [NBP2-79745]

Protein Array: Carboxypeptidase A1/CPA1 Antibody (CPA1/2712) [NBP2-79745] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Carboxypeptidase A1/CPA1 Antibody (CPA1/2712). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.

Applications for Carboxypeptidase A1/CPA1 Antibody (CPA1/2712)

Application
Recommended Usage

Immunohistochemistry

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Carboxypeptidase A1/CPA1

Carboxypeptidase A is a pancreatic exopeptidase. Three different forms of human pancreatic procarboxypeptidase A have been isolated. The A1 and A2 forms are monomeric proteins with different biochemical properties.

Alternate Names

CPA1

Gene Symbol

CPA1

UniProt

Additional Carboxypeptidase A1/CPA1 Products

Product Documents for Carboxypeptidase A1/CPA1 Antibody (CPA1/2712)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Carboxypeptidase A1/CPA1 Antibody (CPA1/2712)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

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Protocols

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