Caspase-8 Antibody - (Pro and Active) - BSA Free

Novus Biologicals | Catalog # NBP1-05123

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunoprecipitation

Cited:

Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Caspase-8 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to the C-terminus of human Caspase-8 (PQPTFTLRKKLVFPSD). This sequence is common to various Caspase-8 forms including: 1. Caspase 8a (aa 481-496 GenBank no. NP_001219.2) 2. Caspase 8b (aa 464-479, GenBank no. NP_203519.1) 3. Caspase 8c (aa 449-464, GenBank no. NP_203520.1) 4. Caspase 8L (aa 523-538, GenBank no. gbAAL87628.1) 5. Truncated caspase 8 (as 251-266, GenBank no. gi3928274)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Caspase-8 Antibody - (Pro and Active) - BSA Free

Western Blot: Caspase-8 Antibody(Pro and Active) [NBP1-05123]

Western Blot: Caspase-8 Antibody(Pro and Active) [NBP1-05123]

Caspase-8-Antibody-Pro-and-Active-Western-Blot-NBP1-05123-img0006.jpg
Immunohistochemistry-Paraffin: Caspase-8 Antibody - (Pro and Active) [NBP1-05123]

Immunohistochemistry-Paraffin: Caspase-8 Antibody - (Pro and Active) [NBP1-05123]

Immunohistochemistry-Paraffin: Caspase-8 Antibody - (Pro and Active) [NBP1-05123] - Formalin-fixed, paraffin-embedded sections from a brain tumor tissue array stained for Caspase-8 expression using NBP1-05123 at 1:2000. A. Anaplastic glioma cores from two different patients, positive (left) and negative (right) staining for caspase-8. B. Higher magnification from the caspase-8 positive (A, left) core.
Western Blot: Caspase-8 Antibody(Pro and Active) [NBP1-05123]

Western Blot: Caspase-8 Antibody(Pro and Active) [NBP1-05123]

Western Blot: Caspase-8 Antibody - (Pro and Active) [NBP1-05123] - AML12 whole cell lysate. Good specific band is observed. Primary antibody at 1: 2000, secondary antibody at 1: 20000. Skimmed milk as blocking buffer. Western blot image submitted by a verified customer review.
Caspase-8 Antibody - (Pro and Active)

Western Blot: Caspase-8 Antibody - (Pro and Active) [NBP1-05123] -

Western Blot: Caspase-8 Antibody - (Pro and Active) [NBP1-05123] - The level of proteins playing essential roles in programmed cell death (Bax, BcL-2, Cyt c, & caspases 3, 8, & 9) & inflammation (TNF-alpha ) in the heart of hypertensive rats (A) SHR & (B) DOCA-salt, & rats after administration of URB597. The expression of examined protein is shown in comparison to the control. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30223427), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Caspase-8 Antibody - (Pro and Active)

Western Blot: Caspase-8 Antibody - (Pro and Active) [NBP1-05123] -

Western Blot: Caspase-8 Antibody - (Pro and Active) [NBP1-05123] - The level of proteins playing essential roles in programmed cell death (Bax, BcL-2, Cyt c, & caspases 3, 8, & 9) & inflammation (TNF-alpha ) in the heart of hypertensive rats (A) SHR & (B) DOCA-salt, & rats after administration of URB597. The expression of examined protein is shown in comparison to the control. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30223427), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Caspase-8 Antibody - (Pro and Active)

Western Blot: Caspase-8 Antibody - (Pro and Active) [NBP1-05123] -

Western Blot: Caspase-8 Antibody - (Pro and Active) [NBP1-05123] - The level of proteins playing essential roles in programmed cell death (Bax, BcL-2, Cyt c, & caspases 3, 8, & 9) & inflammation (TNF-alpha ) in the heart of hypertensive rats (A) SHR & (B) DOCA-salt, & rats after administration of URB597. The expression of examined protein is shown in comparison to the control. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30223427), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Caspase-8 Antibody - (Pro and Active) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:1000-1:5000

Immunoprecipitation

1:50-1:200

Western Blot

1:1000-1:2000

Reviewed Applications

Read 1 review rated 5 using NBP1-05123 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Caspase-8

Apoptosis, or programmed cell death, is a common property of all multicellular organisms. The current dogma of apoptosis suggests that the components of the core cell-death machinery are integral to cells and widely conserved across species. Caspases, a family of cysteinyl aspartate-specific proteases, are integral components of the cell death machinery (reviewed in Siegal, 2006; and Lavrik et al, 2005). They play a central role in the initiation and execution of apoptotic cell death and in inflammation. Caspases are typically divided into 3 major groups, depending on the structure of their prodomain and their function. Group 1: inflammatory caspases (caspases 1, 4, 5, 11, 12, 14). Group II: initiator of apoptosis caspases (caspases 2, 8, 9). Group II: effector caspases (caspases 3, 6, 7). Caspases are constitutively expressed in almost all cell types as inactive proenzymes (zymogens: enzyme precursors which require a biochemical change to become active enzymes) that are processed and activated in response to a variety of pro-apoptotic or inflammatory stimuli. The procaspases (32-56 kDa) contain four domains: an N-terminal prodomain (2-25 kDa), a large subunit (p20: 17-21 kDa), a small subunit (p10: 10-13 kDa) and a short linker region between the large and small subunits. Caspase activation involves proteolytic processing of the proenzyme at specific aspartate residues between the domains. This results in removal of the prodomain as well as the linker region and formation of a heterodimer containing one large and one small subunit (p20-p10). The active caspase is a tetramer composed of two heterodimers (p202-p102). Active caspases mediate cell death and inflammation through cleavage of particular cellular substrates that are involved in these processes. Multiple isoforms of caspase 8 have been identified, including caspase 8a [496 amino acids (aa), GenBank no. NP_001219.2], caspase 8b (479 aa, Gen Bank no. NP_203519.1), caspase 8c (464 aa, GenBank no. NP_203520.1), and caspase 8L (538 aa, GenBank no. gbAAL87628.1). Truncated caspase-8 forms have also been described. Users are encoraged to go to the NCBI ACE View Data Base for more information. http://www.ncbi.nih.gov/IEB/Research/Acembly/ Caspase-8 may be observed on Western blots at varying apparent molecular weights depending on isoform, truncation, or cleavage forms present.

Alternate Names

CASP8, Caspase8, Mch5

Entrez Gene IDs

841 (Human); 12370 (Mouse); 64044 (Rat)

Gene Symbol

CASP8

UniProt

NP_001073595 (Mouse)

Additional Caspase-8 Products

Product Documents for Caspase-8 Antibody - (Pro and Active) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Caspase-8 Antibody - (Pro and Active) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Caspase-8 Antibody - (Pro and Active) - BSA Free

Customer Reviews for Caspase-8 Antibody - (Pro and Active) - BSA Free (1)

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Customer Images

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  • Caspase-8 Antibody - (Pro and Active)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: AML12 whole cell lysate
    Species: Mouse
    Verified Customer | Posted 05/06/2020
    Good specific band is observed. Primary antibody: 1: 2000 secondary antibody: 1: 20000 Skimmed milk as blocking buffer
    Primary antibody: 1: 2000 secondary antibody: 1: 20000
    Caspase-8 Antibody - (Pro and Active) - BSA Free NBP1-05123

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Protocols

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FAQs for Caspase-8 Antibody - (Pro and Active) - BSA Free

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  • Q: May we ask the MW difference between the active form and the proform of Caspase 8 while performing WB?

    A: In treated cells induced to undergo apoptosis, caspase-8 migrates as a 55/53 kDa (pro-form), 41/42 kDa (a cleaved/active or intermediate form), and 18 kDa (active form). The proform (55/53 kDa) is still seen in treated cells because not all cells undergo apoptosis at once.

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Associated Pathways

Apoptosis Signaling Pathway
Apoptosis Signaling Pathway Thumbnail