CBS Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-33518

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CBS Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human CBS. The exact sequence is proprietary.

Localization

Cytoplasm, Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

61 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CBS Antibody - BSA Free

Knockout Validated: CBS Antibody [NBP1-33518]

Western Blot: CBS Antibody [NBP1-33518]

Western Blot: CBS Antibody [NBP1-33518] - Wild-type (WT) and CBS knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Knockdown Validated: CBS Antibody [NBP1-33518]

Western Blot: CBS Antibody [NBP1-33518]

Western Blot: CBS Antibody [NBP1-33518] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody
Western Blot: CBS Antibody [NBP1-33518]

Western Blot: CBS Antibody [NBP1-33518]

Western Blot: CBS Antibody [NBP1-33518] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: CBS Antibody [NBP1-33518]

Immunohistochemistry-Paraffin: CBS Antibody [NBP1-33518]

Immunohistochemistry-Paraffin: CBS Antibody [NBP1-33518] - OVCAR3 xenograft, using CBS antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: CBS Antibody [NBP1-33518]

Western Blot: CBS Antibody [NBP1-33518]

Western Blot: CBS Antibody [NBP1-33518] - Mouse tissue extracts (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody diluted by 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: CBS Antibody [NBP1-33518]

Western Blot: CBS Antibody [NBP1-33518]

Western Blot: CBS Antibody [NBP1-33518] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunoprecipitation: CBS Antibody [NBP1-33518]

Immunoprecipitation: CBS Antibody [NBP1-33518]

Immunoprecipitation: CBS Antibody [NBP1-33518] - CBS antibody immunoprecipitates CBS protein in IP experiments. IP samples: HepG2 whole cell extract. A. 50 ug HepG2 whole cell extract. B. Control with 4 ug of preimmune Rabbit IgG. C. Immunoprecipitation of CBS protein by 4 ug CBS antibody. 7.5 % SDS-PAGE. The immunoprecipitated CBS protein was detected by CBS antibody diluted at 1:500.
CBS Antibody

Immunohistochemistry-Paraffin: CBS Antibody [NBP1-33518] -

Immunohistochemistry-Paraffin: CBS Antibody [NBP1-33518] - CBS antibody detects CBS protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
CBS stained by CBS antibody (NBP1-33518) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
CBS Antibody

Immunocytochemistry/ Immunofluorescence: CBS Antibody [NBP1-33518] -

Immunocytochemistry/ Immunofluorescence: CBS Antibody [NBP1-33518] - CBS antibody detects CBS protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: CBS stained by CBS antibody (NBP1-33518) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Blue: Fluoroshield with DAPI.
Scale bar= 10um.

Applications for CBS Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CBS

The protein encoded by this gene is involved in the transsulfuration pathway. The first step of this pathway, from homocysteine to cystathionine, is catalyzed by this protein. CBS deficiency can cause homocystinuria which affects many organs and tissues, including the eyes and the skeletal, vascular and central nervous systems.

Alternate Names

cystathionine-beta-synthase, EC 4.2.1.2210Beta-thionase

Gene Symbol

CBS

Additional CBS Products

Product Documents for CBS Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for CBS Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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