CD155/PVR Antibody (305) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-90501

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 305 expressed in HEK293

Format

Azide and BSA Free
View all CD155/PVR Primary Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD155/PVR (Accession#: NP_081790.1; Met1-Arg345).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for CD155/PVR Antibody (305) - Azide and BSA Free

Flow Cytometry: CD155/PVR Antibody (305) [NBP2-90501]

Flow Cytometry: CD155/PVR Antibody (305) [NBP2-90501]

Flow Cytometry: CD155/PVR Antibody (305) [NBP2-90501] - Flow cytometric analysis of Mouse CD155/PVR expression on BABL/c splenocytes. Cells were stained with purified anti-Mouse CD155/PVR, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
CD155/PVR Antibody (305) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: CD155/PVR Antibody (305) [NBP2-90501] -

Immunocytochemistry/Immunofluorescence: CD155/PVR Antibody (305) [NBP2-90501] - Immunofluorescence staining of Mouse CD155/PVR in raw264.7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with NBP2-90501 antibody (1:100) at 4C overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated (left panel, captured by laser confocal scanning microscope; right panel, captured by fluorescence microscope) Goat Anti-rabbit IgG Secondary antibody, counterstained with DAPI (blue). Positive staining was localized to plasma membrane.

Applications for CD155/PVR Antibody (305) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:25-1:100

Immunocytochemistry/ Immunofluorescence

1:50-1:1000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD155/PVR

Polio Virus Receptor (PVR) is a member of Ig-superfamily with 3 Ig-domains in arrangement V-C-C. Four mRNAs are produced from single gene for PVR, (mapped to human chromosome band 19q13.1-13.2). Two of the mRNAs (called H20A and H20B or alpha and delta) encode cell surface molecules of about 43kDa and 45kDa polypeptide backbone, respectively. Two other splice variants, beta and gamma encode secreted molecules of about 44kDa. The cytoplasmic tails are short (aa 35-50) and are rich in serine phosphorylated residues.

Long Name

Poliovirus Receptor

Alternate Names

CD155, HVED, Necl-5, PVR, PVS

Gene Symbol

PVR

Additional CD155/PVR Products

Product Documents for CD155/PVR Antibody (305) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for CD155/PVR Antibody (305) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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