CD177 Antibody (4C4) - Azide and BSA Free

Novus Biologicals | Catalog # H00057126-M01

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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4C4

Format

Azide and BSA Free
View all CD177 Primary Antibodies »

Product Specifications

Immunogen

CD177 (AAH29167, 21 a.a. ~ 120 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ALLCQFGTVQHVWKVSDLPRQWTPKNTSCDSGLGCQDTLMLIESGPQVSLVLSKGCTEAKDQEPRVTEHRMGPGLSLISYTFVCRQEDFCNNLVNSLPLW

Specificity

CD177 (4C4)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Novus Biologicals Mouse CD177 Antibody (4C4) - Azide and BSA Free (H00057126-M01) is a monoclonal antibody validated for use in IHC, WB, ELISA and IP. Anti-CD177 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for CD177 Antibody (4C4) - Azide and BSA Free

Western Blot: CD177 Antibody (4C4) [H00057126-M01]

Western Blot: CD177 Antibody (4C4) [H00057126-M01]

Western Blot: CD177 Antibody (4C4) [H00057126-M01] - Analysis of CD177 expression in transfected 293T cell line by CD177 monoclonal antibody (M01), clone 4C4.Lane 1: CD177 transfected lysate(46.4 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: CD177 Antibody (4C4) [H00057126-M01]

Immunohistochemistry-Paraffin: CD177 Antibody (4C4) [H00057126-M01]

Immunohistochemistry-Paraffin: CD177 Antibody (4C4) [H00057126-M01] - Analysis of monoclonal antibody to CD177 on formalin-fixed paraffin-embedded human lymph node. Antibody concentration 3 ug/ml.
Immunoprecipitation: CD177 Antibody (4C4) [H00057126-M01]

Immunoprecipitation: CD177 Antibody (4C4) [H00057126-M01]

Immunoprecipitation: CD177 Antibody (4C4) [H00057126-M01] - Analysis of CD177 transfected lysate using anti-CD177 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CD177 MaxPab rabbit polyclonal antibody.
ELISA: CD177 Antibody (4C4) [H00057126-M01]

ELISA: CD177 Antibody (4C4) [H00057126-M01]

ELISA: CD177 Antibody (4C4) [H00057126-M01] - Detection limit for recombinant GST tagged CD177 is approximately 3ng/ml as a capture antibody.

Applications for CD177 Antibody (4C4) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CD177

NB1, a glycosyl-phosphatidylinositol (GPI)-linked N-glycosylated cell surface glycoprotein, was first described in a case of neonatal alloimmune neutropenia (Lalezari et al., 1971 [PubMed 5552408]).[supplied by OMIM]

Long Name

Polycythemia Rubra Vera Protein 1

Alternate Names

CD177, HNA-2a, NB1 GP, PRV1

Entrez Gene IDs

57126 (Human)

Gene Symbol

CD177

OMIM

162860 (Human)

UniProt

AAH29167

Additional CD177 Products

Product Documents for CD177 Antibody (4C4) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CD177 Antibody (4C4) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Neutrophils

Citations for CD177 Antibody (4C4) - Azide and BSA Free

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Protocols

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FAQs

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