CD21 Antibody (1F8)

Novus Biologicals | Catalog # NBP1-22527

Clone 1F8 was used by HLDA to establish CD designation.
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Key Product Details

Species Reactivity

Human, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1F8
View all CD21 Primary Antibodies »

Product Specifications

Immunogen

Purified receptor for the C3d fragment of the third complement component CR2, from human tonsil.

Reactivity Notes

Monkey reactivity reported from a verified customer review.

Localization

Cell membrane

Marker

Histiocytic Marker

Specificity

This CD21 Antibody (1F8) reacts with CD21 antigen of MW 145 kDa, present on the surface of mature B cells. The antigen is present at high density on follicular dendritic cells. CD21 is involved in transmitting growth-promoting signals to the interior of the B cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Novus Biologicals Mouse CD21 Antibody (1F8) (NBP1-22527) is a monoclonal antibody validated for use in IHC. Anti-CD21 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for CD21 Antibody (1F8)

Immunohistochemistry-Paraffin: CD21 Antibody (1F8) [NBP1-22527]

Immunohistochemistry-Paraffin: CD21 Antibody (1F8) [NBP1-22527]

Immunohistochemistry-Paraffin: CD21 Antibody (1F8) [NBP1-22527] - Human tonsil tissue section stained with CD21 antibody. IHC-P image submitted by a verified customer review.
Immunohistochemistry-Paraffin: CD21 Antibody (1F8) [NBP1-22527]

Immunohistochemistry-Paraffin: CD21 Antibody (1F8) [NBP1-22527]

Immunohistochemistry-Paraffin: CD21 Antibody (1F8) [NBP1-22527] - Formalin fixed paraffin embedded human tonsil stained with CD21 antibody (NBP1-22527).

Applications for CD21 Antibody (1F8)

Application
Recommended Usage

Immunohistochemistry

1:10-1:25

Immunohistochemistry-Paraffin

1:10-1:25
Application Notes
IHC-P: recommended pretreatment of HistoZyme. Recommended incubation time of 30 min at RT.

Reviewed Applications

Read 2 reviews rated 4.5 using NBP1-22527 in the following applications:

Formulation, Preparation, and Storage

Purification

Tissue culture supernatant

Formulation

Tissue culture supernatant

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: CD21

CD21 also known as complement receptor 2 (CR2), C3d receptor or EBV receptor is a 140 kDa protein. CD21 is a glycosylated type I transmembrane protein consisting of an extracellular face of a series of 15 or 16 CCP domains. CD21 is the receptor for complement components C3d and iC3b as well as the Epstein-Barr virus (EBV) glycoprotein gp350/220. The soluble CD21 (sCD21) was shown to efficiently trigger CD23 signaling pathways in human monocytes. By inducing release of proinflammatory cytokines and upregulating expression of molecules involved in antigen presentation, sCD21 modulates critical monocyte functions that may be relevant to allergic and inflammatory disorders.

Alternate Names

C3DR, CD21, CR2

Entrez Gene IDs

1380 (Human)

Gene Symbol

CR2

UniProt

P20023 (Human)

Additional CD21 Products

Product Documents for CD21 Antibody (1F8)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CD21 Antibody (1F8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CD21 Antibody (1F8)

Customer Reviews for CD21 Antibody (1F8) (2)

4.5 out of 5
2 Customer Ratings
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Customer Images

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  • CD21 Antibody (1F8)
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: human tonsil
    Species: Human
    Verified Customer | Posted 01/27/2021
    CD21 on human tonsil
    CD21 Antibody (1F8) NBP1-22527
  • CD21 Antibody (1F8)
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: cynomolgus monkey spleen
    Species: Cynomolgus Monkey
    Verified Customer | Posted 01/27/2021
    CD21_1:10_cynomolgus monkey spleen
    1:10 & 1:25 dilutions were tested on human tonsil and cynomolgus monkey spleen and tonsil tissue. An anti-mouse polymer was used for detection. The human tonsil stained nicely, but there was no staining in the monkey tissues.
    CD21 Antibody (1F8) NBP1-22527

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