CD96 Antibody (BLR065G)
Novus Biologicals | Catalog # NBP3-14682
Recombinant Monoclonal
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit Clone # BLR065G
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Product Specifications
Immunogen
residues 29-502 (ECD)
Clonality
Monoclonal
Host
Rabbit
Description
Novus Biologicals Rabbit CD96 Antibody (BLR065G) (NBP3-14682) is a recombinant monoclonal antibody validated for use in IHC, WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for CD96 Antibody (BLR065G)
Immunoprecipitation: CD96 Antibody (BLR065G) [NBP3-14682]
Immunoprecipitation: CD96 Antibody [NBP3-14682] - Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from MOLT-4 cellsprepared using NETN lysis buffer. Antibodies: Rabbit antiCD96 recombinant monoclonal antibody used for IP at 6 ul per reaction. CD96 wasalso immunoprecipitated by a previous lot of this antibody. For blotting immunoprecipitated CD96,was used at 1:1000. Chemiluminescence withan exposure time of 75 seconds.ICC/IF: CD96 Antibody [NBP3-14682] -
ICC/IF: CD96 Antibody [NBP3-14682] -: Rabbit anti-CD96 recombinant monoclonalantibody [BLR065G]. Secondary: HRP conjugated goat anti-rabbit IgG. Substrate:DAB.Immunohistochemistry-Paraffin: CD96 Antibody [NBP3-14682] -
Immunohistochemistry-Paraffin: CD96 Antibody [NBP3-14682] -: Rabbit anti-CD96 recombinant monoclonalantibody [BLR065G]. Secondary: HRP conjugated goat anti-rabbit IgG Substrate:DAB.Immunohistochemistry-Paraffin: CD96 Antibody [NBP3-14682] -
Immunohistochemistry-Paraffin: CD96 Antibody [NBP3-14682] -Rabbit anti-CD96 recombinant monoclonal antibody[BLR065G]. Secondary: HRP-conjugatedgoat anti-rabbit IgG. Substrate: DAB.Western Blot: CD96 Antibody [NBP3-14682] -
Western Blot: CD96 Antibody [NBP3-14682] -Whole cell lysate (10 µg) from HeLa, MOLT-4, HEK293T,A2058, Jurkat, CCRF-CEM, Hep-G2, KG-1, and HH cellsprepared using NETN lysis buffer. Antibody: Rabbit antiCD96 recombinant monoclonal antibody used at 1:1000. Secondary: HRPconjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 minutes.Applications for CD96 Antibody (BLR065G)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100 to 1:500
Immunohistochemistry
1:100 to 1:500
Immunohistochemistry-Paraffin
1:100 to 1:500
Immunoprecipitation
6 µl/1 mg lysate
Western Blot
1:1000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Formulation, Preparation, and Storage
Purification
>95%
Formulation
Borate Buffered Saline (BBS) pH 8.2 with, BSA Free
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 2 - 8 C / 1 year from date of receipt
Background: CD96
Alternate Names
CD96, TACTILE
Gene Symbol
CD96
Additional CD96 Products
Product Documents for CD96 Antibody (BLR065G)
Product Specific Notices for CD96 Antibody (BLR065G)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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