CD97 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-86935

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CD97 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: RDSKTSSAEVTIQNVIKLVDELMEAPGDVEALAPPVRHLIATQLLSNLEDIMRILAKSLPKGPFTYISPSNTELTLMIQERGDKNVTMGQ

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24949957).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit CD97 Antibody - BSA Free (NBP1-86935) is a polyclonal antibody validated for use in IHC and WB. Anti-CD97 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for CD97 Antibody - BSA Free

CD97 Antibody - BSA Free Immunohistochemistry-Paraffin: CD97 Antibody - BSA Free [NBP1-86935]

Immunohistochemistry-Paraffin: CD97 Antibody - BSA Free [NBP1-86935]

Analysis in human lymph node and skeletal muscle tissues Corresponding ADGRE5 RNA-seq data are presented for the same tissues.
CD97 Antibody - BSA Free Immunohistochemistry-Paraffin: CD97 Antibody - BSA Free [NBP1-86935]

Immunohistochemistry-Paraffin: CD97 Antibody - BSA Free [NBP1-86935]

Staining of human lymph node shows strong membranous positivity in non-germinal center cells.
CD97 Antibody - BSA Free Immunohistochemistry-Paraffin: CD97 Antibody - BSA Free [NBP1-86935]

Immunohistochemistry-Paraffin: CD97 Antibody - BSA Free [NBP1-86935]

Staining of human skeletal muscle shows no positivity in myocytes as expected.
CD97 Antibody - BSA Free Immunohistochemistry-Paraffin: CD97 Antibody - BSA Free [NBP1-86935]

Immunohistochemistry-Paraffin: CD97 Antibody - BSA Free [NBP1-86935]

Staining of human rectum shows strong cytoplasmic positivity in glandular cells.
CD97 Antibody - BSA Free Immunohistochemistry-Paraffin: CD97 Antibody - BSA Free [NBP1-86935]

Immunohistochemistry-Paraffin: CD97 Antibody - BSA Free [NBP1-86935]

Staining of human placenta shows strong positivity in microvilli in trophoblastic cells.

Applications for CD97 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
WB reported in scientific literature (PMID: 24949957). For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD97

CD97, a CD55 Receptor, is a leukocyte antigen located on most activated leukocytes. CD97 is highly homologous to EMR2 with five EGF-like domains in the N-terminal extracellular domain, suggesting that it may be involved in cell signaling as well as in cell adhesion (Hamann et al., 1996). Two variants are produced by alternative splicing, where the shorter form lacks exons 5 and 6 and reduces the number of EGF-like domains from 5 to 3, compared to the full-length isoform. CD97 is associated with with the activated lesions of multiple sclerosis. CD97 expression is also implicated in the dedifferentiation of colon and thyroid tumors. CD97 is expressed abundantly in cells of hematopoietic origin and is markedly upregulated on activated T and B cells. ESTs have been isolated from a wide variety of tissue libraries.

Alternate Names

CD97

Gene Symbol

ADGRE5

Additional CD97 Products

Product Documents for CD97 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for CD97 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CD97 Antibody - BSA Free

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Protocols

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