Chordin-like 1/CHRDL1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-12874

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rat (93%), Rhesus Macaque (99%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Chordin-like 1/CHRDL1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Chordin-like 1/CHRDL1. The exact sequence is proprietary.

Reactivity Notes

Chicken (80%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for Chordin-like 1/CHRDL1 Antibody - BSA Free

Immunohistochemistry-Paraffin: Chordin-like 1/CHRDL1 Antibody [NBP3-12874]

Immunohistochemistry-Paraffin: Chordin-like 1/CHRDL1 Antibody [NBP3-12874]

Immunohistochemistry-Paraffin: Chordin-like 1/CHRDL1 Antibody [NBP3-12874] - Chordin-like 1/CHRDL1 antibody detects Chordin-like 1/CHRDL1 protein at cytosol on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Chordin-like 1/CHRDL1 antibody (NBP3-12874) dilution: 1:500. Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15min
Immunohistochemistry-Paraffin: Chordin-like 1/CHRDL1 Antibody [NBP3-12874]

Immunohistochemistry-Paraffin: Chordin-like 1/CHRDL1 Antibody [NBP3-12874]

Immunohistochemistry-Paraffin: Chordin-like 1/CHRDL1 Antibody [NBP3-12874] - Chordin-like 1/CHRDL1 antibody detects Chordin-like 1/CHRDL1 protein at cytosol on mouse hind brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse hind brain. Chordin-like 1/CHRDL1 antibody (NBP3-12874) dilution: 1:500. Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15min
Chordin-like 1/CHRDL1 Antibody

Western Blot: Chordin-like 1/CHRDL1 Antibody [NBP3-12874] -

Western Blot: Chordin-like 1/CHRDL1 Antibody [NBP3-12874] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Chordin-like 1/CHRDL1 antibody (NBP3-12874) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Chordin-like 1/CHRDL1 Antibody

Immunocytochemistry/ Immunofluorescence: Chordin-like 1/CHRDL1 Antibody [NBP3-12874] -

Immunocytochemistry/ Immunofluorescence: Chordin-like 1/CHRDL1 Antibody [NBP3-12874] - Chordin-like 1/CHRDL1 antibody detects Chordin-like 1/CHRDL1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Chordin-like 1/CHRDL1 stained by Chordin-like 1/CHRDL1 antibody (NBP3-12874) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Blue: Fluoroshield with DAPI.
Scale bar= 10um.

Applications for Chordin-like 1/CHRDL1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Chordin-like 1/CHRDL1

CHRDL1 encodes an antagonist of bone morphogenetic protein 4. The encoded protein may play a role in topographic retinotectal projection and in the regulation of retinal angiogenesis in response to hypoxia. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq]

Alternate Names

CHRDL1, CRDL1, Neuralin 1, NRLN1, Ventroptin alpha, VOPT

Gene Symbol

CHRDL1

Additional Chordin-like 1/CHRDL1 Products

Product Documents for Chordin-like 1/CHRDL1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Chordin-like 1/CHRDL1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

BMP Family

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Protocols

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FAQs

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