Claudin-1 Antibody (2S2H8)

Name: Claudin-1 Antibody (2S2H8)
Brand: Novus Biologicals
SKU: NBP3-57393
Price: 529 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-57393

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunoprecipitation, Knockdown Validated

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2S2H8

View all Claudin-1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide. This information is considered to be commercially sensitive. (Swissprot# O95832)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is highly specific and optimized for use in Immunohistochemistry.

Normal Tissues Tested
Fallopian tube, Pancreas, Lung, Kidney, Placenta, Stomach, Liver, Thyroid, Appendix, Testis, Prostate, Tonsil, Cervix, esophagus, Breast, Colon, Brain

Cancer Tissues Tested
Lung adenocarcinoma, Lung squamous carcinoma, Hepatocellular Carcinoma, Hepatocholangio carcinoma, Thyroid Cancer, Prostate cancer, cervical squamous cell carcinoma, Breast Cancer, Colon Cancer

Positive Controls
Liver, tonsil (Squamous epithelial cells), esophagus (Squamous epithelial cells), Pancreas (Exocrine glandular cells), Hepatocellular Carcinoma, Thyroid Cancer, Colon Cancer

Negative Controls
Brain, tonsil (lymphocyte), Pancreas (Pancreatic endocrine cells)

Applications for Claudin-1 Antibody (2S2H8)

Application

Recommended Usage

ELISA

1 ug/mL

Immunohistochemistry-Paraffin

1:100 - 1:400

Immunoprecipitation

0.5ug-4ug antibody for 200ug-400ug extracts of whole cells

Western Blot

1:1000 - 1:5000

Application Notes

For immunofluorescence on paraffin-embedded tissues, the recommended concentration is 1:50 - 1:200

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 50% glycerol, 0.05% BSA, (pH 7.3)

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Claudin-1

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome.

Alternate Names

Claudin1, CLD1, CLDN1, SEMP1

Gene Symbol

CLDN1

Additional Claudin-1 Products

Product Documents for Claudin-1 Antibody (2S2H8)

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Certificate of Analysis

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Product Specific Notices for Claudin-1 Antibody (2S2H8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.