cleavage stimulation factor Antibody - BSA Free

Novus Biologicals | Catalog # NB100-60443

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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Predicted:

Orangutan (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all cleavage stimulation factor Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 75 and 125 of human cleavage stimulation factor, 3' pre-RNA, subunit 1, 50kDa using the numbering given in entry NP_001315.1 (GeneID 1477 ).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for cleavage stimulation factor Antibody - BSA Free

Western Blot: cleavage stimulation factor Antibody [NB100-60443]

Western Blot: cleavage stimulation factor Antibody [NB100-60443]

Western Blot: cleavage stimulation factor Antibody [NB100-60443] - Detection of human and mouse CSTF50 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CSTF50 antibody NB100-60443 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry-Paraffin: cleavage stimulation factor Antibody [NB100-60443]

Immunohistochemistry-Paraffin: cleavage stimulation factor Antibody [NB100-60443]

Immunohistochemistry-Paraffin: cleavage stimulation factor Antibody [NB100-60443] - FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CSTF50 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: cleavage stimulation factor Antibody [NB100-60443]

Immunohistochemistry-Paraffin: cleavage stimulation factor Antibody [NB100-60443]

Immunohistochemistry-Paraffin: cleavage stimulation factor Antibody [NB100-60443] - Detection of mouse CSTF50 by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-CSTF50 (NB100-60443). Detection: DAB
Immunoprecipitation: cleavage stimulation factor Antibody [NB100-60443]

Immunoprecipitation: cleavage stimulation factor Antibody [NB100-60443]

Immunoprecipitation: cleavage stimulation factor Antibody [NB100-60443] - Detection of human CSTF50 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Hela cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CSTF50 antibody NB100-60443 used for IP at 6 ug per reaction. CSTF50 was also immunoprecipitated by another rabbit anti-CSTF50 antibody. For blotting immunoprecipitated CSTF50, NB100-60443 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for cleavage stimulation factor Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:5000

Immunohistochemistry-Paraffin

1:1000 - 1:5000

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:1000 - 1:5000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Reviewed Applications

Read 1 review rated 5 using NB100-60443 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: cleavage stimulation factor

This gene encodes one of three subunits which combine to form cleavage stimulation factor (CSTF). CSTF is involved in the polyadenylation and 3'end cleavage of pre-mRNAs. Similar to mammalian G protein beta subunits, this protein contains transducin-like repeats. Several transcript variants with different 5' UTR, but encoding the same protein, have been found for this gene.

Alternate Names

CF-1 50 kDa subunit, Cleavage stimulation factor 50 kDa subunit, cleavage stimulation factor subunit 1, cleavage stimulation factor, 3' pre-RNA, subunit 1, 50kD, cleavage stimulation factor, 3' pre-RNA, subunit 1, 50kDa, CSTF 50 kDa subunit, CstF-50, CstFp50

Entrez Gene IDs

1477 (Human)

Gene Symbol

CSTF1

UniProt

NP_001315.1 (Human)

Additional cleavage stimulation factor Products

Product Documents for cleavage stimulation factor Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for cleavage stimulation factor Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for cleavage stimulation factor Antibody - BSA Free

Customer Reviews for cleavage stimulation factor Antibody - BSA Free (1)

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Hela whole cell lysate
    Species: Human
    Verified Customer | Posted 05/22/2015
    cleavage stimulation factor Antibody - BSA Free NB100-60443

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