Collagen II Antibody (5B2.5) - BSA Free

Western Blot: Collagen II Antibody (5B2.5) - BSA Free [NB600-844] -

Expression levels of related genes during differentiation induction in the 2D environment. (a) qRT-PCR analysis of Sox9, ACAN & collagen II on days 7, 14 & 21 during induction. (b) qRT-PCR analysis of collagen X, Runx2 & annexin V on days 7, 14 & 21 during induction. The results were normalized to B2M mRNA expression. Values are means ± SD (n = 3). (c) Expression of ACAN, collagen II, collagen X & Runx2 was detected by western blotting on day 10 during induction. (d) Expression of ACAN, collagen II, collagen X & Runx2 was detected by western blotting on day 21 during induction. Significant differences from the control group (non-transfection cells) are indicated by *p < 0.05 or **p < 0.01; differences between IHH & SHH transfection groups are indicated by #p < 0.05 or ##p < 0.01 Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30858866), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Collagen II Antibody (5B2.5) - BSA Free [NB600-844] -

Expression levels of related genes during differentiation induction in the RCCS environment. (a) qPCR analysis of Sox9, ACAN & collagen II on days 7, 14 & 21 during induction. (b) qRT-PCR analysis of collagen X, Runx2, & annexin V on days 7, 14 & 21 during induction. The results were normalized to B2M mRNA expression. Values are means ± SD (n = 3). (c) Expression of ACAN, collagen II, collagen X & Runx2 was detected by western blotting on day 10 during differentiation induction. (d) Expression of ACAN, collagen II, collagen X & Runx2 was detected by western blotting on day 21 during differentiation induction. Significant differences from the control group (non-transfection cells) are indicated by *p < 0.05 or ** p < 0.01; differences between IHH & SHH transfection groups are indicated by #p < 0.05 or ##p < 0.01 Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30858866), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: Collagen II Antibody (5B2.5) - BSA Free [NB600-844] -

Immunohistochemistry: Collagen II Antibody (5B2.5) - BSA Free [NB600-844] - Staining results for the process of differentiation induction in the RCCS environment. (a) Cellular morphology. (b) Toluidine blue staining on cell-microcarrier complexes. (c) Toluidine blue staining on cells after inoculation onto slides. (d) Collagen II immunohistochemical staining on cell-microcarrier complexes. (e) Collagen II immune-histochemical staining on cells after inoculation onto slides. (f) Annexin V-Cy3 immunofluorescence staining. (Bar = 100 um) Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30858866), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: Collagen II Antibody (5B2.5) - BSA Free [NB600-844] -

Immunohistochemistry: Collagen II Antibody (5B2.5) - BSA Free [NB600-844] - Staining results for the process of differentiation induction in the 2D environment. (a) Toluidine blue staining; (b) Collagen II immunohistochemical staining; (c) Annexin V-Cy3 immunofluorescence staining. (Bar = 100 um) Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30858866), licensed under a CC-BY license. Not internally tested by Novus Biologicals.