Complement C3a Antibody (K13/16-5.7) - BSA Free

Novus Biologicals | Catalog # NBP1-05122

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA Capture, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # K13/16-5.7

Format

BSA Free
View all Complement C3a Primary Antibodies »

Product Specifications

Immunogen

Complement C3a Antibody (K13/16-5.7) - BSA Free was developed against Human C3a.

Epitope

No reaction is seen with a synthetic octapeptide representing the C3a C-terminal.

Specificity

Complement C3a Antibody (K13/16-5.7) - BSA Free is specific for human C3, C3a and C3a (desArg) and does not cross-react with C4a or C5a.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Concentration for any given batch will be between 0.85 - 1.15 mg/mL.

Scientific Data Images for Complement C3a Antibody (K13/16-5.7) - BSA Free

Immunohistochemistry: Complement C3a Antibody (K13/16-5.7) - BSA Free [NBP1-05122]

Immunohistochemistry: Complement C3a Antibody (K13/16-5.7) - BSA Free [NBP1-05122]

Immunohistochemistry: Complement C3a Antibody (K13/16-5.7) - BSA Free [NBP1-05122] - Staining in human kidney. Strong staining of plasma of nephritis kidney was seen. Dilution: 1:75.
Western Blot: Complement C3a Antibody (K13/16-5.7)BSA Free [NBP1-05122]

Western Blot: Complement C3a Antibody (K13/16-5.7)BSA Free [NBP1-05122]

Western Blot: Complement C3a Antibody (K13/16-5.7) - BSA Free [NBP1-05122] - Lane 1: purified C3, lane 2: purifed C3 reduced/DTT, lane 3: purified C3a, lane 4: EDTA-plasma. Lane 5: zymosan-activated serum
Immunohistochemistry-Paraffin: Complement C3a Antibody (K13/16-5.7) - BSA Free [NBP1-05122]

Immunohistochemistry-Paraffin: Complement C3a Antibody (K13/16-5.7) - BSA Free [NBP1-05122]

Immunohistochemistry-Paraffin: Complement C3a Antibody (K13/16-5.7) - BSA Free [NBP1-05122] - Staining of human kidney. Strong staining of veins of nephritis kidney was seen. Dilution: 1:150.
Sandwich ELISA Capture: Complement C3a Antibody (K13/16-5.7)BSA Free [NBP1-05122]

Sandwich ELISA Capture: Complement C3a Antibody (K13/16-5.7)BSA Free [NBP1-05122]

Sandwich ELISA Capture: Complement C3a Antibody (K13/16-5.7) - BSA Free [NBP1-05122] - The calibration curve of a sandwich assay for Complement C3a using NBP1-05122 as the capture antibody and NBP2-23496 as the biotinylated detection antibody.

Applications for Complement C3a Antibody (K13/16-5.7) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:75

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:100-1:2000
Application Notes
ELISA: NBP1-05122 can be used as a capture antibody in sandwich ELISA with NBP2-23497 as a biotinylated detection antibody. (4, 5) NBP1-05122 can also be used to immunopurify C3a (3). NBP1-05122 effectively inhibits the biological activity of C3a in a guinea-pig platelet activation assay. (2) WB: NBP1-05122 was used in Western blotting. (2, 3, 5) IHC: NBP1-05122 was used in IHC in formaline fixed paraffin embedded tissue with a dilution of 1:150. IF: GAU 103-16 was used in IF with a dilution of 1:75.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM Phosphate (pH 7.4) and 0.5M NaCl

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Complement C3a

The complement system, or complement cascade, is a part of the innate immune system that assists in defense against pathogens (1). Complement C3, also called C3 or C3 protein, is one of nine complement proteins and is the main component of the complement system which is composed of over 30 soluble and membrane-bound proteins (1). C3 is cleaved by C3 convertase into two fragments, the smaller C3a and larger C3b (1,2). Human C3a is 77 amino acids (aa) in length and has a theoretical molecular weight of ~9 kDa (2). Structurally, complement C3a consists of four anti-parallel helical structures with three disulfide bridges (2). The C3a fragment binds the G-protein coupled receptor (GPCR) C3a (C3aR) (2,3). Functionally, C3a plays an important role in regulating both adaptive immunity and innate immunity (1-3).

The C3a/C3aR pathway is often referred to as a "double-edged sword" because it has both anti-inflammatory and pro-inflammatory functions (2,3). For instance, its anti-inflammatory role includes pathogen elimination and reduced interferon-gamma (IFN-gamma) expression and cytotoxicity in natural killer cells (2). By contrast, complement C3a is an anaphylatoxin and plays a role in inflammation by prompting histamine release from mast cells and increasing permeability of the vascular system (2-4). The C3a/C3aR pathway has also been indicated in a number of diseases including Alzheimer's disease, asthma, and kidney disease (3). Patients with various kidney diseases tend to show higher levels of complement C3a in the plasma and urine (3).

References

1. Mathern, D. R., & Heeger, P. S. (2015). Molecules Great and Small: The Complement System. Clinical Journal of the American Society of Nephrology: CJASN. https://doi.org/10.2215/CJN.06230614

2. Gao, S., Cui, Z., & Zhao, M. H. (2020). The Complement C3a and C3a Receptor Pathway in Kidney Diseases. Frontiers in Immunology. https://doi.org/10.3389/fimmu.2020.01875

3. Ricklin, D., Reis, E. S., Mastellos, D. C., Gros, P., & Lambris, J. D. (2016). Complement component C3 - The "Swiss Army Knife" of innate immunity and host defense. Immunological Reviews. https://doi.org/10.1111/imr.12500

4. Haddad, A., & Wilson, A. M. (2020). Biochemistry, Complement. In StatPearls. StatPearls Publishing.

Alternate Names

Acylation Stimulating Protein, acylation-stimulating protein cleavage product, AHUS5, ARMD9, ASP, C3, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, C3a, C3a anaphylatoxin, C3adesArg, C3b, C3bc, C3-beta-c, complement C3, Complement C3 alpha chain, Complement C3 beta chain, Complement C3b alpha' chain, Complement C3c alpha' chain fragment 1, Complement C3c alpha' chain fragment 2, Complement C3d fragment, Complement C3dg fragment, Complement C3f fragment, Complement C3g fragment, complement component 3, complement component C3, complement component C3a, complement component C3b, CPAMD1, EC 3.4.21.43, epididymis secretory sperm binding protein Li 62p, HEL-S-62p, prepro-C3

Gene Symbol

C3

Additional Complement C3a Products

Product Documents for Complement C3a Antibody (K13/16-5.7) - BSA Free

Certificate of Analysis

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Product Specific Notices for Complement C3a Antibody (K13/16-5.7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Complement C3a Antibody (K13/16-5.7) - BSA Free

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Protocols

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