Complement C4 Antibody

Novus Biologicals | Catalog # NBP3-12295

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Rat, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit
View all Complement C4 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide taken within amino acid region 1220-1270 on human complement C4-A preprotein.

Reactivity Notes

Baboon (100%). Rat (80%).

Clonality

Polyclonal

Host

Rabbit

Scientific Data Images for Complement C4 Antibody

Immunohistochemistry-Paraffin: Complement C4 Antibody [NBP3-12295]

Immunohistochemistry-Paraffin: Complement C4 Antibody [NBP3-12295]

Immunohistochemistry-Paraffin: Complement C4 Antibody [NBP3-12295] - Baboon liver. 1:100 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification.
Immunohistochemistry-Paraffin: Complement C4 Antibody [NBP3-12295]

Immunohistochemistry-Paraffin: Complement C4 Antibody [NBP3-12295]

Immunohistochemistry-Paraffin: Complement C4 Antibody [NBP3-12295] - Baboon liver. 1:100 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification.
Immunohistochemistry-Paraffin: Complement C4 Antibody [NBP3-12295]

Immunohistochemistry-Paraffin: Complement C4 Antibody [NBP3-12295]

Immunohistochemistry-Paraffin: Complement C4 Antibody [NBP3-12295] - Baboon kidney. 1:100 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification.

Applications for Complement C4 Antibody

Application
Recommended Usage

ELISA

1:10000

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:100

Immunoprecipitation

1:200

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Contains Tris, HCl/glycine buffer (pH 7.4-7.8), 30% glycerol, 0.5% BSA, along with cryo-protective agents, and HEPES (exact storage and stabilization buffer information is proprietary).

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.

Background: Complement C4

C4 consists of three polypeptide chains (alpha, molecular weight 95 kDa, beta, molecular weight 75 kDa and gamma, molecular weight 33 kDa) and is usually present in serum at 200-600mg/L. It is a protein of the classical complement pathway. When activated by C1s, a peptide C4a is released and the generated C4b binds C2 which is in turn cleaved by C1s to produce the classical pathway C3 convertase, namely C4b2a. C4b also has opsonin activity and binds to macrophages and neutrophils via their CR1 receptor.

Alternate Names

C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2, C4, complement component 4A (Rodgers blood group), RG

Gene Symbol

C4A

Additional Complement C4 Products

Product Documents for Complement C4 Antibody

Certificate of Analysis

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Product Specific Notices for Complement C4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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