Complement C4d Antibody (7H4)

Novus Biologicals | Catalog # NBP3-11291

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Guinea Pig

Applications

Immunohistochemistry-Frozen, Western Blot, Immunoassay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 7H4
View all Complement C4d Primary Antibodies »

Product Specifications

Immunogen

The monoclonal antibody 7H4 (also known as clone M4d2) recognizes an epitope on the alpha 2 chain of human C4d.

Specificity

The monoclonal antibody 7H4 recognizes C4, C4b and C4d.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Complement C4d Antibody (7H4)

Application
Recommended Usage

Immunoassay

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
For Immunohistochemistry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 um filtered solution in PBS, 0.1% BSA

Preservative

0.02% Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Complement C4d

The monoclonal antibody 7H4 (also known as clone M4d2) recognizes an epitope on the alpha 2 chain of human C4d. Complement factor C4 (MW 41 kDa), formerly known as Gg protein, consists of an alpha-, beta- and gamma-chain. The classical pathway of complement and the mannose binding lectin (MBL) activation pathway converge at C4. Activated C1, MASP-1 and MASP-2 cleave C4 resulting in the formation of C4a and C4b. The latter can be cleaved by factor I resulting in C4c and C4d, in which step all functional sites are lost. The C4d activation fragment of C4 is an excellent marker for classical complement pathway and MBL pathway activation. The thioester formed between the side chains of Cys1010 and Gln1013 within the C4d region of the alpha-chain mediates covalent attachment to the target surface bearing activated forms of C1s or MASP. Furthermore, C4d is highly homologous to C3d with over 35% shared amino acid sequence. In a number of diseases such as rheumatoid arthritis (RA), hereditary angioedema (HAE), systemic lupus erythematosus (SLE) and chronic urticaria with hypercomplementemia levels of C4d are significantly elevated in serum or plasma. C4d levels may also be elevated in plasma from patients with a variety of humoral autoimmune diseases in which complement activation is known to occur. Deposition of C4d in peritubular capillaries has been shown to be a sensitive marker for antibody-mediated (humoral) rejection in renal transplant biopsies. The monoclonal antibody 7H4 recognizes C4, C4b and C4d. Antibody 7H4 cross reacts with guinea pig.

Alternate Names

C4, C4A, C4A2, C4A3, C4A4, C4A6, C4S, CO4, complement C4A, CPAMD2, RG

Gene Symbol

C4A

Additional Complement C4d Products

Product Documents for Complement C4d Antibody (7H4)

Certificate of Analysis

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Product Specific Notices for Complement C4d Antibody (7H4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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