Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 22

Format

BSA Free
View all Complement Component C9 Primary Antibodies »

Product Specifications

Immunogen

Complement factor C9

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Novus Biologicals Mouse Complement Component C9 Antibody (22) - BSA Free (NBP2-21612) is a monoclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Complement Component C9 Antibody (22) - BSA Free

Immunohistochemistry: Complement Component C9 Antibody (22) - BSA Free [NBP2-21612]

Immunohistochemistry: Complement Component C9 Antibody (22) - BSA Free [NBP2-21612]

Immunohistochemistry: Complement Component C9 Antibody (22) - BSA Free [NBP2-21612] - Staining of human liver with complement component C9 antibody. Plasma of portal veins are strongly stained.
Immunohistochemistry: Complement Component C9 Antibody (22) - BSA Free [NBP2-21612]

Immunohistochemistry: Complement Component C9 Antibody (22) - BSA Free [NBP2-21612]

Immunohistochemistry: Complement Component C9 Antibody (22) - BSA Free [NBP2-21612] - Staining of human kidney with complement component C9 antibody. Plasma of veins are strongly stained.
Immunohistochemistry: Complement Component C9 Antibody (22) - BSA Free [NBP2-21612]

Immunohistochemistry: Complement Component C9 Antibody (22) - BSA Free [NBP2-21612]

Immunohistochemistry: Complement Component C9 Antibody (22) - BSA Free [NBP2-21612] - staining of tonsil tissue with complement component C9 antibody. Follicular dendritic cell network stained strongly in germinal centers of tonsil tissue.
Immunohistochemistry: Complement Component C9 Antibody (22) - BSA Free [NBP2-21612]

Immunohistochemistry: Complement Component C9 Antibody (22) - BSA Free [NBP2-21612]

Immunohistochemistry: Complement Component C9 Antibody (22) - BSA Free [NBP2-21612] - staining of tonsil tissue with complement component C9 antibody. Follicular dendritic cell network stained strongly in germinal centers of tonsil tissue. Also moderate staining were observed from plasma of veins.

Applications for Complement Component C9 Antibody (22) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
ELISA: NBP2-21612 recognizes C9 in human serum diluted 1/50 in Tris buffer (20 mM Tris-base, 1 mM MgCl2, 1 mM CaCl2 and 140 mM NaCl) and incubated for 2 hours at 37C using a human IgM coated (10 ug/mL overnight at 4C, blocked with PBS 7.2 + 1% BSA for 1 hour) ELISA plate.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Complement Component C9

Complement factor C9 is a 71kDa single-chain ellipsoid a-globular molecule of 558 amino acids including a leader sequence of 20 amino acids. C9 bind the C5b8 complex on membranes and has an unique property in forming tubular structures containing between 12 and 18 monomers, closely resembling the membrane attack complex (MAC). The primary site of synthesis is hepatocytes, secondary sites are monocytes, fibroblasts and glial cells. The normal level of C9 in plasma is 60 ug/mL, and the level is up regulated from hepatocytes by IFN-gamma.

Alternate Names

complement component 9, complement component C9

Gene Symbol

C9

Additional Complement Component C9 Products

Product Documents for Complement Component C9 Antibody (22) - BSA Free

Certificate of Analysis

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Product Specific Notices for Complement Component C9 Antibody (22) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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