COP9 signalosome complex subunit 2 Antibody - BSA Free
Novus Biologicals | Catalog # NB100-363
![Western Blot: COP9 signalosome complex subunit 2 Antibody [NB100-363]](https://resources.rndsystems.com/images/products/COP9-signalosome-complex-subunit-2-Antibody-Western-Blot-NB100-363-img0011.jpg "Western Blot: COP9 signalosome complex subunit 2 Antibody [NB100-363]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse
Predicted:
Rat (100%), Xenopus (100%), Zebrafish (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residues 1 and 50 of human COP9 constitutive photomorphogenic homolog subunit 2 using the numbering given in entry NP_004227.1 (GeneID 9318).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for COP9 signalosome complex subunit 2 Antibody - BSA Free
Western Blot: COP9 signalosome complex subunit 2 Antibody [NB100-363]
Western Blot: COP9 signalosome complex subunit 2 Antibody [NB100-363] - Detection of Human CSN2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CSN2 antibody NB100-363 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.![Immunohistochemistry-Paraffin: COP9 signalosome complex subunit 2 Antibody [NB100-363]](https://resources.rndsystems.com/images/products/COP9-signalosome-complex-subunit-2-Antibody-Immunohistochemistry-Paraffin-NB100-363-img0009.jpg "Immunohistochemistry-Paraffin: COP9 signalosome complex subunit 2 Antibody [NB100-363]")
Immunohistochemistry-Paraffin: COP9 signalosome complex subunit 2 Antibody [NB100-363]
Immunohistochemistry-Paraffin: COP9 signalosome complex subunit 2 Antibody [NB100-363] - Section of human prostate carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-CSN2 used at a dilution of 1:5,000 (0.2ug/ml) and 1:1,000 (1ug/ml). Detection: DABApplications for COP9 signalosome complex subunit 2 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:1000 - 1:5000
Western Blot
1:1000-1:10000
Application Notes
By Western blot, this antibody recognizes a band at ~50 kDa, representing CSN2 protein. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: COP9 signalosome complex subunit 2
Alternate Names
ALIEN, Alien homolog, COP9 constitutive photomorphogenic homolog subunit 2 (Arabidopsis), COP9 signalosome complex subunit 2, CSN2JAB1-containing signalosome subunit 2, SGN2TR-interacting protein 15, Signalosome subunit 2, thyroid receptor interacting protein 15, Thyroid receptor-interacting protein 15, TRIP15TRIP-15
Entrez Gene IDs
9318 (Human)
Gene Symbol
COPS2
UniProt
Additional COP9 signalosome complex subunit 2 Products
Product Documents for COP9 signalosome complex subunit 2 Antibody - BSA Free
Certificate of Analysis
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Product Specific Notices for COP9 signalosome complex subunit 2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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