COPS6 Antibody (OTI4E7)

Immunohistochemistry: COPS6 Antibody (OTI4E7) [NBP2-46333]

Immunohistochemistry: COPS6 Antibody (OTI4E7) [NBP2-46333] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Western Blot: COPS6 Antibody (OTI4E7) [NBP2-46333] -

CSN6 is required for the proliferation, migration and invasion of melanoma cells and regulates cell cycle progression.A qRT-PCR assays and western blot analysis of CSN6 expression in CSN6-knockdown A375 and MV3 melanoma cells. B Growth curves of A375 and MV3 cells expressing shGFP, shCSN6#1, or shCSN6#2 (n = 4). C Representative fluorescence micrographs and quantification of BrdU staining to detect the amount of DNA synthesis in CSN6-knockdown A375 and MV3 melanoma cells. Scale bar = 100 μm. D Flow cytometry assays performed to quantify the cell population in each phase of the cell cycle. A375 and MV3 cells expressing shGFP, shCSN6#1, or shCSN6#2 were evaluated. E, F Migration and invasion assays performed with A375 and MV3 cells expressing shGFP, shCSN6#1, or shCSN6#2 (left), and calculations of the migratory or invasive cells (right). Migratory cells were stained with crystal violet and counted at 12 h. Invasive cells were stained and counted at 24 h. Scale bar = 50 μm. G Protein expression of several cell cycle regulatory proteins and metastasis-related proteins in A375 and MV3 cells expressing shGFP, shCSN6#1, or shCSN6#2. All data are shown as the mean +/- SD, and data were analyzed using two-tailed Student’s t-tests; *P < 0.05, **P < 0.01, and ***P < 0.001. All P-values are based on control versus treatment comparisons. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33483464), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: COPS6 Antibody (OTI4E7) [NBP2-46333] -

CSN6 recovery rescues the cell proliferation, migration, and invasion of CSN6-silenced melanoma cells.A qRT-PCR and western blot assays were used to confirm CSN6 expression in CSN6-rescued CSN6-knockdown A375 and MV3 melanoma cells. B CSN6 recovery rescued the proliferation of A375 and MV3 melanoma cells. Growth curves are shown for the CSN6-rescued CSN6-knockdown cells (n = 4). C Representative fluorescence micrographs and quantification of BrdU staining in CSN6-rescued CSN6-knockdown A375 and MV3 cells are shown. Scale bar = 100 μm. D, E Migration and invasion assays were performed with CSN6-rescued CSN6-knockdown A375 and MV3 cells (left), and the quantification of migratory or invasive cells (right). Migratory cells were stained with crystal violet and counted at 12 h. Invasive cells were stained and counted at 24 h. Scale bar = 50 μm. F Western blot assays were used to detect the protein expression levels of cell cycle regulatory proteins and metastasis-related proteins in CSN6-rescued CSN6-knockdown A375 and MV3 melanoma cells. All data are shown as the mean +/- SD, *P < 0.05 and **P < 0.01. All P-values are based on control versus treatment comparisons. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33483464), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: COPS6 Antibody (OTI4E7) [NBP2-46333] -

The effects induced by CSN6 silencing were abrogated by downregulation of UBR5 expression in CSN6-knockdown cells.A Total cellular extracts from CSN6-knockdown and UBR5-knockdown CSN6-knockdown A375 and MV3 cells were prepared and subjected to western blotting with the indicated antibody. B The migration and invasion of CSN6-knockdown and UBR5-knockdown CSN6-knockdown A375 and MV3 cells were quantified. C The growth curves of CSN6-knockdown and UBR5-knockdown CSN6-knockdown A375 and MV3 cells are shown (n = 4). D An in vitro colony formation assay was performed with CSN6-knockdown and UBR5-knockdown CSN6-knockdown A375 and MV3 cells. E, F Stably transfected CSN6-knockdown or UBR5-knockdown CSN6-knockdown A375 cells (1 × 106 cells) or control cells were subcutaneously injected into right dorsal side of female nude mice (n = 6). Tumor volume was measured when the tumors reached a certain volume. Two weeks after injection, the mice were sacrificed, and the tumors were collected and weighed. One-way ANOVA. G Immunohistochemical analysis was conducted to detect CSN6, UBR5, CDK9 and Ki-67 expression, Scale bar = 100 μm. H The model of the CSN6-UBR5-CDK9 axis is shown. All data are shown as the mean +/- SD, *P < 0.05, **P < 0.01, and ***P < 0.001. All P-values are based on control versus treatment comparisons. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33483464), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: COPS6 Antibody (OTI4E7) [NBP2-46333] -

High CSN6 expression is associated with a poor melanoma patient prognosis.A Representative immunohistochemical staining assays showing CSN6 expression in human benign nevus tissue (left, n = 9) and melanoma tissue (right, n = 15). B Immunohistochemical analyses of CSN6 expression levels in 9 benign nevus tissue samples and 15 melanoma tissue samples, P < 0.001. C, D Western blot and qRT-PCR assays performed to detect CSN6 expression in the A375, MV3, and Skmel28 melanoma cell lines and the immortalized melanocyte cell line PIG1. E, F Kaplan–Meier analysis of the overall survival probability using data from the online R2 database. P-values calculated by the log-rank test are indicated. G Box plot analysis of CSN6 expression levels with the pathological stage of melanoma. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33483464), licensed under a CC-BY license. Not internally tested by Novus Biologicals.