CPT1A Antibody

Novus Biologicals | Catalog # NB100-53791

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all CPT1A Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-DPAQTVEQRLKLFK corresponding to internal region according to NP_001867.2, NP_001027017.1.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25026599). Rat reactivity reported in scientific literature (PMID: 23045433).

Specificity

This antibody is expected to recognize both reported isoforms (NP_001867.2; NP_001027017.1) but is not expected to recognise CPT1B.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

88 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Goat CPT1A Antibody (NB100-53791) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-CPT1A Antibody: Cited in 9 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for CPT1A Antibody

Western Blot: CPT1A Antibody [NB100-53791]

Western Blot: CPT1A Antibody [NB100-53791]

CPT1A-Antibody-Western-Blot-NB100-53791-img0002.jpg
Western Blot: CPT1A Antibody [NB100-53791]

Western Blot: CPT1A Antibody [NB100-53791]

Western Blot: CPT1A Antibody [NB100-53791] - Staining of MCF7 cell lysate (35 ug protein in RIPA buffer). Antibody at 2 ug/mL. Detected by chemiluminescence.
CPT1A Antibody

Immunohistochemistry-Paraffin: CPT1A Antibody [NB100-53791] -

Immunohistochemistry-Paraffin: CPT1A Antibody [NB100-53791] - Negative Control showing staining of paraffin embedded Human Liver, with no primary antibody.
CPT1A Antibody

Immunohistochemistry-Paraffin: CPT1A Antibody [NB100-53791] -

Immunohistochemistry-Paraffin: CPT1A Antibody [NB100-53791] - (5ug/ml) staining of paraffin embedded Human Liver. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Applications for CPT1A Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

5-7 ug/ml

Peptide ELISA

Detection limit 1:128000

Western Blot

1-3 ug/mL
Application Notes
Western blot: Approx 80-85 kDa band observed in lysates of cell line MCF7 (calculated MW of 86.2 kDa according to NP_001027017.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CPT1A

The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation.

Long Name

Carnitine O-palmitoyltransferase 1, liver isoform

Alternate Names

CPT I, CPT1, CPT1-L, CPTI-L, EC 2.3.1.21

Entrez Gene IDs

1374 (Human)

Gene Symbol

CPT1A

UniProt

P50416 (Human)

Additional CPT1A Products

Product Documents for CPT1A Antibody

Certificate of Analysis

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Product Specific Notices for CPT1A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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