CREB3 Antibody (3H5) - Azide and BSA Free

Novus Biologicals | Catalog # H00010488-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3H5

Format

Azide and BSA Free
View all CREB3 Primary Antibodies »

Product Specifications

Immunogen

CREB3 (NP_006359, 273 a.a. ~ 371 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DPYQLELPALQSEVPKDSTHQWLDGSDCVLQAPGNTSCLLHYMPQAPSAEPPLEWPFPDLFSEPLCRGPILPLQANLTRKGGWLPTGSPSVILQDRYSG

Reactivity Notes

Human. Other species not tested.

Specificity

CREB3 - cAMP responsive element binding protein 3

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for CREB3 Antibody (3H5) - Azide and BSA Free

Western Blot: CREB3 Antibody (3H5) [H00010488-M01]

Western Blot: CREB3 Antibody (3H5) [H00010488-M01]

Western Blot: CREB3 Antibody (3H5) [H00010488-M01] - Analysis of CREB3 expression in transfected 293T cell line by CREB3 monoclonal antibody (M01), clone 3H5.Lane 1: CREB3 transfected lysate(41.4 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: CREB3 Antibody (3H5) [H00010488-M01]

Immunohistochemistry-Paraffin: CREB3 Antibody (3H5) [H00010488-M01]

Immunohistochemistry-Paraffin: CREB3 Antibody (3H5) [H00010488-M01] - Analysis of monoclonal antibody to CREB3 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.
Western Blot: CREB3 Antibody (3H5) [H00010488-M01]

Western Blot: CREB3 Antibody (3H5) [H00010488-M01]

Western Blot: CREB3 Antibody (3H5) [H00010488-M01] - Analysis of CREB3 over-expressed 293 cell line, cotransfected with CREB3 Validated Chimera RNAi ( Cat # H00010488-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CREB3 monoclonal antibody (M01), clone 3H5 (Cat # H00010488-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Sandwich ELISA: CREB3 Antibody (3H5) [H00010488-M01] - Detection limit for recombinant GST tagged CREB3 is approximately 0.1ng/ml as a capture antibody.

Applications for CREB3 Antibody (3H5) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin), RNAi validation, and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CREB3

This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds to the cAMP-responsive element, an octameric palindrome. The protein interacts with host cell factor C1, which also associates with the herpes simplex virus (HSV) protein VP16 that induces transcription of HSV immediate-early genes. This protein and VP16 both bind to the same site on host cell factor C1. It is thought that the interaction between this protein and host cell factor C1 plays a role in the establishment of latency during HSV infection. An additional transcript variant has been identified, but its biological validity has not been determined.

Alternate Names

basic leucine zipper protein, cAMP responsive element binding protein 3, cAMP responsive element binding protein 3 (luman), cAMP-responsive element-binding protein 3, CREB-3, cyclic AMP response element (CRE)-binding protein/activating transcriptionfactor 1, cyclic AMP-responsive element-binding protein 3, Luman, LZIPMGC15333, MGC19782, Transcription factor LZIP-alpha

Entrez Gene IDs

10488 (Human)

Gene Symbol

CREB3

OMIM

606443 (Human)

UniProt

NP_006359 (Human)

Additional CREB3 Products

Product Documents for CREB3 Antibody (3H5) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for CREB3 Antibody (3H5) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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