CSTF3 Antibody (1D4) - Azide and BSA Free

Novus Biologicals | Catalog # H00001479-M01

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1D4

Format

Azide and BSA Free
View all CSTF3 Primary Antibodies »

Product Specifications

Immunogen

CSTF3 (AAH09792, 1 a.a. ~ 103 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSGDGATEQAAEYVPEKVKKAEKKLEENPYDLDAWSILIREAQNQPIDKARKTYERLVAQFPSSGRFWKLYIEAEVTILFYFFLYQYCSIHCSDRKQVRNIAN

Localization

Nuclear

Specificity

CSTF3 - cleavage stimulation factor, 3' pre-RNA, subunit 3, 77kDa

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for CSTF3 Antibody (1D4) - Azide and BSA Free

Western Blot: CSTF3 Antibody (1D4) [H00001479-M01]

Western Blot: CSTF3 Antibody (1D4) [H00001479-M01]

Western Blot: CSTF3 Antibody (1D4) [H00001479-M01] - Analysis of CSTF3 expression in transfected 293T cell line by CSTF3 monoclonal antibody (M01), clone 1D4. Lane 1: CSTF3 transfected lysate(11.44 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: CSTF3 Antibody (1D4) [H00001479-M01]

Immunocytochemistry/ Immunofluorescence: CSTF3 Antibody (1D4) [H00001479-M01]

Immunocytochemistry/Immunofluorescence: CSTF3 Antibody (1D4) [H00001479-M01] - Analysis of monoclonal antibody to CSTF3 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: CSTF3 Antibody (1D4) [H00001479-M01]

Immunohistochemistry-Paraffin: CSTF3 Antibody (1D4) [H00001479-M01]

Immunohistochemistry-Paraffin: CSTF3 Antibody (1D4) [H00001479-M01] - Analysis of monoclonal antibody to CSTF3 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.
Western Blot: CSTF3 Antibody (1D4) [H00001479-M01]

Western Blot: CSTF3 Antibody (1D4) [H00001479-M01]

Western Blot: CSTF3 Antibody (1D4) [H00001479-M01] - Analysis of CSTF3 over-expressed 293 cell line, cotransfected with CSTF3 Validated Chimera RNAi ( Cat # H00001479-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CSTF3 monoclonal antibody (M01), clone 1D4 (Cat # H00001479-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Sandwich ELISA: CSTF3 Antibody (1D4) [H00001479-M01] - Detection limit for recombinant GST tagged CSTF3 is approximately 0.1ng/ml as a capture antibody.

Applications for CSTF3 Antibody (1D4) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against recombinant protein and transfected lysate for WB. It has been used for IF, IHC-P, RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CSTF3

The protein encoded by this gene is one of three (including CSTF1 and CSTF2) cleavage stimulation factors that combine to form the cleavage stimulation factor complex (CSTF). This complex is involved in the polyadenylation and 3' end cleavage of pre-mRNAs. The encoded protein functions as a homodimer and interacts directly with both CSTF1 and CSTF2 in the CSTF complex. Alternative splicing results in multiple transcript variants encoding different isoforms.

Alternate Names

CF-1 77 kDa subunit, Cleavage stimulation factor 77 kDa subunit, cleavage stimulation factor subunit 3, cleavage stimulation factor, 3' pre-RNA, subunit 3, 77kD, cleavage stimulation factor, 3' pre-RNA, subunit 3, 77kDa, CSTF 77 kDa subunit, CstF-77, MGC117398, MGC43001, MGC75122

Entrez Gene IDs

1479 (Human)

Gene Symbol

CSTF3

OMIM

600367 (Human)

UniProt

AAH09792 (Human)

Additional CSTF3 Products

Product Documents for CSTF3 Antibody (1D4) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CSTF3 Antibody (1D4) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CSTF3 Antibody (1D4) - Azide and BSA Free

Customer Reviews for CSTF3 Antibody (1D4) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review CSTF3 Antibody (1D4) - Azide and BSA Free and earn rewards!

Have you used CSTF3 Antibody (1D4) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies