Cyclin E1 Antibody (MSVA-898R)

Novus Biologicals | Catalog # NBP3-57245

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # MSVA-898R
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Product Specifications

Immunogen

Recombinant human Cyclin E1 protein fragment Swissprot P24864

Localization

Nucleus

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is highly specific and optimized for use in Immunohistochemistry.

It has been validated on TMAs covering 76 normal tissues and numerous cancer tissues.

The antibody concentration is optimized for dilution within dilution range using commercially available antibody diluent for immunohistochemistry.

Positive Control
Postive control:Placenta: A strong Cyclin E1 staining should be seen in most cytotrophoblast cells..

Negative Control
Negitve control:Normal kidney: Cyclin E1 staining should be largely absent in tubular and glomerular cells.

Storage
Antibody can be stored at 4degC. For extended storage, aliquot and store at -20degC. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Cyclin E1 Antibody (MSVA-898R)

Cyclin E1 Antibody (MSVA-898R)

Immunohistochemistry-Paraffin:Cyclin E1 Antibody (MSVA-898R)NBP3-57245

Mature placenta showing a strong Cyclin E1 staining of most cytotrophoblast cells
Cyclin E1 Antibody (MSVA-898R)

Immunohistochemistry-Paraffin:Cyclin E1 Antibody (MSVA-898R)NBP3-57245

Ovarian clear cell carcinoma with strong Cyclin E1 staining of all tumor cells
Cyclin E1 Antibody (MSVA-898R)

Immunohistochemistry-Paraffin:Cyclin E1 Antibody (MSVA-898R)NBP3-57245

Skin with a gradually increasing Cyclin E1 staining of squamous epithelial cells towards the surface cell layers
Cyclin E1 Antibody (MSVA-898R) Cyclin E1 Antibody (MSVA-898R) Cyclin E1 Cancer Tissue Gallery

Cyclin E1 Cancer Tissue Gallery

Cyclin E1 Antibody (MSVA-898R) Cyclin E1 Antibody (MSVA-898R) Cyclin E1 Cancer Tissue Gallery

Cyclin E1 Cancer Tissue Gallery

Cyclin E1 Antibody (MSVA-898R) Cyclin E1 Antibody (MSVA-898R) Cyclin E1 Normal Tissue Gallery

Cyclin E1 Normal Tissue Gallery

Cyclin E1 Antibody (MSVA-898R) Cyclin E1 Antibody (MSVA-898R) Cyclin E1 Normal Tissue Gallery

Cyclin E1 Normal Tissue Gallery

Cyclin E1 Antibody (MSVA-898R) Cyclin E1 Antibody (MSVA-898R) Cyclin E1 Normal Tissue Gallery

Cyclin E1 Normal Tissue Gallery

Cyclin E1 Antibody (MSVA-898R) Cyclin E1 Antibody (MSVA-898R) Cyclin E1 Normal Tissue Gallery

Cyclin E1 Normal Tissue Gallery

Cyclin E1 Antibody (MSVA-898R) Cyclin E1 Antibody (MSVA-898R) Cyclin E1 Normal Tissue Gallery

Cyclin E1 Normal Tissue Gallery

Cyclin E1 Antibody (MSVA-898R) Cyclin E1 Antibody (MSVA-898R) Cyclin E1 Normal Tissue Gallery

Cyclin E1 Normal Tissue Gallery

Applications for Cyclin E1 Antibody (MSVA-898R)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:50 1:100
Application Notes
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:50 at 37C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Preservative

<0.1% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Cyclin E1

Alternate Names

CCNE, CCNE1

Gene Symbol

CCNE1

Additional Cyclin E1 Products

Product Documents for Cyclin E1 Antibody (MSVA-898R)

Certificate of Analysis

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Product Specific Notices for Cyclin E1 Antibody (MSVA-898R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

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Protocols

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