Cytokeratin 17 Antibody (23GB6535)

Novus Biologicals | Catalog # NBP3-51399

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB6535
View all Cytokeratin 17 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human Cytokeratin 17 (Uniprot: Q04695)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cytokeratin 17 Antibody (23GB6535)

Cytokeratin 17 Antibody (23GB6535)

Immunocytochemistry/ Immunofluorescence: Cytokeratin 17 Antibody (23GB6535)[NBP3-51399] -

Immunocytochemical staining of HeLa cells with Cytokeratin 17 antibody (1:1,000). Nuclei were stained blue with DAPI; Cytokeratin 17 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
Cytokeratin 17 Antibody (23GB6535)

Flow Cytometry: Cytokeratin 17 Antibody (23GB6535)[NBP3-51399] -

Validation of Cytokeratin 17 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Cytokeratin 17 antibody (1:2,000) and analyzed using BD flow cytometer.
Cytokeratin 17 Antibody (23GB6535)

Flow Cytometry: Cytokeratin 17 Antibody (23GB6535)[NBP3-51399] -

Flow cytometric analysis of Cytokeratin 17expression in HeLa cells using Cytokeratin 17 antibody (1:2,000). Green, isotype control; red, Keratin 17.

Applications for Cytokeratin 17 Antibody (23GB6535)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Cytokeratin 17

Among the members of the cytokeratin (CK) subfamily of intermediate filament (IF) proteins, CK 17 is normally expressed in the basal cells of complex epithelia but not in stratified or simple epithelia. It has an unusual expression pattern in normal and diseased states and has potential importance in tumor diagnosis. CK 17 synthesis seems to be a marker of basal cell differentiation in complex epithelia and therefore indicative of a certain type of epithelial "stem cells" (1). It has been shown that CK 17 regulates cell growth through binding to the adaptor protein 14-3-3sigma. These findings reveal a new and unexpected role for the intermediate filament cytoskeleton in influencing cell growth and size by regulating protein synthesis (2). CK 17 is expressed in the nail bed, hair follicle, sebaceous glands, and other epidermal appendages (3).

Long Name

Cytokeratin 17/KRT17

Alternate Names

CK-17, cytokeratin-17, K17, keratin 17, type I, keratin, type I cytoskeletal 17, PCHC1

Gene Symbol

KRT17

Additional Cytokeratin 17 Products

Product Documents for Cytokeratin 17 Antibody (23GB6535)

Certificate of Analysis

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Product Specific Notices for Cytokeratin 17 Antibody (23GB6535)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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