Cytokeratin 19 Antibody (A53-B/A2.26 + BA17)
Novus Biologicals | Catalog # NBP2-34272
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Lambda/IgG1 Kappa Clone # A53-B/A2.26 + BA17
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Product Specifications
Immunogen
Human breast cancer MCF-7 cells (A53-B/A2); Human mammary epithelial organoids (BA17)
Localization
Cytoplasmic
Marker
Pancreatic Stem Cell Marker
Specificity
This monoclonal antibody reacts with the rod domain of human cytokeratin-19 (CK19), a polypeptide of 40kDa. CK19 is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50%-60% of follicular carcinomas are also labeled. Anti-CK19 is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Lambda/IgG1 Kappa
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34548)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Cytokeratin 19 Antibody (A53-B/A2.26 + BA17)
![Western Blot: Cytokeratin 19 Antibody (A53-B/A2.26 + BA17) [NBP2-34272]](https://resources.rndsystems.com/images/products/Cytokeratin-19-Antibody-A53-B-A2-26-+-BA17-Western-Blot-NBP2-34272-img0002.jpg "Western Blot: Cytokeratin 19 Antibody (A53-B/A2.26 + BA17) [NBP2-34272]")
Western Blot: Cytokeratin 19 Antibody (A53-B/A2.26 + BA17) [NBP2-34272]
Western Blot: Cytokeratin 19 Antibody (A53-B/A2.26 + BA17) [NBP2-34272] - Western Blot Analysis of HepG2 cell lysate Cytokeratin 19 Antibody (A53-B/A2.26 + BA17).![Immunohistochemistry: Cytokeratin 19 Antibody (A53-B/A2.26 + BA17) [NBP2-34272]](https://resources.rndsystems.com/images/products/Cytokeratin-19-Antibody-A53-B-A2-26-+-BA17-Immunohistochemistry-NBP2-34272-img0003.jpg "Immunohistochemistry: Cytokeratin 19 Antibody (A53-B/A2.26 + BA17) [NBP2-34272]")
Immunohistochemistry: Cytokeratin 19 Antibody (A53-B/A2.26 + BA17) [NBP2-34272]
Immunohistochemistry: Cytokeratin 19 Antibody (A53-B/A2.26 + BA17) [NBP2-34272] - Human breast cancer tissue stained with CK19 antibody. IHC-Fr image submitted by a verified customer review.. Using the DyLight 405 format of this antibody.![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (A53-B/A2.26 + BA17) [NBP2-34272]](https://resources.rndsystems.com/images/products/Cytokeratin-19-Antibody-A53-B-A2-26-+-BA17-Immunohistochemistry-Paraffin-NBP2-34272-img0001.jpg "Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (A53-B/A2.26 + BA17) [NBP2-34272]")
Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (A53-B/A2.26 + BA17) [NBP2-34272]
Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (A53-B/A2.26 + BA17) [NBP2-34272] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with Cytokeratin 19 MAb (A53-B/A2.26 + BA17)Applications for Cytokeratin 19 Antibody (A53-B/A2.26 + BA17)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Cytokeratin 19
Additional Cytokeratin 19 Products
Product Documents for Cytokeratin 19 Antibody (A53-B/A2.26 + BA17)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Cytokeratin 19 Antibody (A53-B/A2.26 + BA17)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
