Cytokeratin 5 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-32470

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (93%), Rat (90%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Cytokeratin 5 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: VSFRSGGSRSFSTASAITPSVSRTSFTSVS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cytokeratin 5 Antibody - BSA Free

Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody [NBP2-32470]

Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody [NBP2-32470]

Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody [NBP2-32470] - Analysis in human skin and pancreas tissues using NBP2-32470 antibody. Corresponding KRT5 RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: Cytokeratin 5 Antibody [NBP2-32470]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 5 Antibody [NBP2-32470]

Immunocytochemistry/Immunofluorescence: Cytokeratin 5 Antibody [NBP2-32470] - Staining of human cell line hTCEpi shows localization to intermediate filaments. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody [NBP2-32470]

Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody [NBP2-32470]

Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody [NBP2-32470] - Staining of human Skin shows strong cytoplasmic positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody [NBP2-32470]

Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody [NBP2-32470]

Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody [NBP2-32470] - Staining of human Liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody [NBP2-32470]

Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody [NBP2-32470]

Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody [NBP2-32470] - Staining of human Pancreas shows no positivity in exocrine glandular cells as expected.
Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody [NBP2-32470]

Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody [NBP2-32470]

Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody [NBP2-32470] - Staining of human Small intestine shows no positivity in glandular cells as expected.

Applications for Cytokeratin 5 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cytokeratin 5

Keratins are a family of structurally related proteins that form the intermediate filament cytoskeleton in epithelial cells. The 58-kD keratin CK-5 is highly similar to other type II keratins and less similar to type I keratins and other intermediate filament proteins. The 58-kD keratin is regulated by retinoids in several tissues and is one of four keratins abundantly expressed in epidermal keratinocytes, where it may be important in maintaining structural integrity of the integument (1). Keratin 5 (CK-5) mRNA and protein are shown to be expressed in normal mammary epithelial cells in culture and are absent from tumor-derived cell lines. This makes CK-5 an important marker in the tumorigenic process, distinguishing normal from tumor cells, and decreased CK-5 expression correlates with tumorigenic progression (2). Dowling-Degos disease (DDD) is an autosomal dominant genodermatosis characterized by progressive and disfiguring reticulate hyperpigmentation of the flexures. Loss of function of CK-5 suggests a crucial role for keratins in the organization of cell adhesion, melanosome uptake, organelle transport, and nuclear anchorage (3).

Alternate Names

CK-5, DDD, EBS2, Keratin 5, KRT5

Gene Symbol

KRT5

Additional Cytokeratin 5 Products

Product Documents for Cytokeratin 5 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Cytokeratin 5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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