Cytosolic Sulfotransferase 2A1/SULT2A1 Antibody (OTI4D7) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-71326

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4D7

Format

Azide and BSA Free
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Product Specifications

Immunogen

Full length human recombinant protein of human SULT2A1 (NP_003158) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

33.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images

Western Blot: Cytosolic Sulfotransferase 2A1/SULT2A1 Antibody (OTI4D7)Azide and BSA Free [NBP2-71326]

Western Blot: Cytosolic Sulfotransferase 2A1/SULT2A1 Antibody (OTI4D7)Azide and BSA Free [NBP2-71326]

Western Blot: Cytosolic Sulfotransferase 2A1/SULT2A1 Antibody (OTI4D7) - Azide and BSA Free [NBP2-71326] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SULT2A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SULT2A1.
Immunocytochemistry/ Immunofluorescence: Cytosolic Sulfotransferase 2A1/SULT2A1 Antibody (OTI4D7) - Azide and BSA Free [NBP2-71326]

Immunocytochemistry/ Immunofluorescence: Cytosolic Sulfotransferase 2A1/SULT2A1 Antibody (OTI4D7) - Azide and BSA Free [NBP2-71326]

Immunocytochemistry/Immunofluorescence: Cytosolic Sulfotransferase 2A1/SULT2A1 Antibody (OTI4D7) - Azide and BSA Free [NBP2-71326] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SULT2A1.
Flow Cytometry: Cytosolic Sulfotransferase 2A1/SULT2A1 Antibody (OTI4D7) - Azide and BSA Free [NBP2-71326]

Flow Cytometry: Cytosolic Sulfotransferase 2A1/SULT2A1 Antibody (OTI4D7) - Azide and BSA Free [NBP2-71326]

Flow Cytometry: Cytosolic Sulfotransferase 2A1/SULT2A1 Antibody (OTI4D7) - Azide and BSA Free [NBP2-71326] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-SULT2A1 antibody, and then analyzed by flow cytometry.
Western Blot: Cytosolic Sulfotransferase 2A1/SULT2A1 Antibody (OTI4D7)Azide and BSA Free [NBP2-71326]

Western Blot: Cytosolic Sulfotransferase 2A1/SULT2A1 Antibody (OTI4D7)Azide and BSA Free [NBP2-71326]

Western Blot: Cytosolic Sulfotransferase 2A1/SULT2A1 Antibody (OTI4D7) - Azide and BSA Free [NBP2-71326] - Analysis of extracts (35ug) from 9 different cell lines by using anti-SULT2A1 monoclonal antibody.

Applications

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000-2000

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cytosolic Sulfotransferase 2A1/SULT2A1

Dehydroepiandrosterone (DHEA) is a major secretory product of the human adrenal cortex during intrauterine development as well as during adulthood. DHEA circulates in the bloodstream as DHEA-sulfate (DHEAS). DHEA has been implicated in a wide range of physiological roles including aging, immunology, learning and memory and obesity.

Alternate Names

DHEA-ST, DHEAS, hSTa, ST2A3, SULT2A1

Gene Symbol

SULT2A1

Additional Cytosolic Sulfotransferase 2A1/SULT2A1 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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