D-Dimer Antibody (28) - BSA Free

Novus Biologicals | Catalog # NBP1-05045

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Canine

Cited:

Human

Applications

Validated:

Immunohistochemistry-Paraffin, Western Blot, ELISA

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 28

Format

BSA Free
View all D-dimer Primary Antibodies »

Product Specifications

Immunogen

Purified human D-dimer

Epitope

The epitope appears to relate to the neo-epitope formed by cleavage of D-dimer from fibrin.

Specificity

Binds human D-dimer. Cross-reacts with directly coated D-monomer but not with directly coated fibrinogen.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Concentration for any given batch will be between 0.85 - 1.15 mg/mL.

Scientific Data Images for D-Dimer Antibody (28) - BSA Free

ELISA: D-Dimer Antibody (28) - BSA Free [NBP1-05045]

ELISA: D-Dimer Antibody (28) - BSA Free [NBP1-05045]

ELISA: D-Dimer Antibody (28) - BSA Free [NBP1-05045] - The calibration curve of a sandwich assay for D-Dimer using NBP1-05045 as capture antibody and NBP2-23592 as the biotinylated detection antibody.

Applications for D-Dimer Antibody (28) - BSA Free

Application
Recommended Usage

ELISA

1:8000

Western Blot

1:100-1:2000
Application Notes
ELISA: NBP1-05045 binds free D-dimer in solution. NBP1-05045 works as a capture antibody in sandwich ELISA with biotinylated NBP1-05045 or NBP2-21616 as a detection antibody. Biotinylated NBP1-05045 works as a detection antibody in sandwich ELISA with NBP2-21616 or NBP1-05045 as a capture antibody. The combination with NBP1-05045 as a capture antibody and NBP2-23592 as a detection antibody is recommended. WB: Due to reducing conditions in Western blotting NBP1-05045 binds the beta-chain (40kDa) of D-Dimer, and not the entire D-Dimer complex. Use in IHC-P reported in scientific literature (PMID:33480806)

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: D-dimer

D-dimer is a fibrin degradation product, a small protein fragment present in the blood after a blood clot is degraded by fibrinolysis.

Long Name

D-Dimer breakdown product of Fibrinogen

Alternate Names

Ddimer, FDP

Additional D-dimer Products

Product Documents for D-Dimer Antibody (28) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for D-Dimer Antibody (28) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for D-Dimer Antibody (28) - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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