DAGLA Antibody

Novus Biologicals | Catalog # NBP1-20905

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all DAGLA Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-PAKQDELVISAR corresponding to C-Terminus according to NP_006124.1.

Epitope

C terminus

Reactivity Notes

This batch is not expected to cross-react with Mouse and Rat in Western blot.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for DAGLA Antibody

Western Blot: DAGLA Antibody [NBP1-20905]

Western Blot: DAGLA Antibody [NBP1-20905]

Western Blot: DAGLA Antibody [NBP1-20905] - Staining of Human Liver lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: DAGLA Antibody [NBP1-20905]

Immunohistochemistry-Paraffin: DAGLA Antibody [NBP1-20905]

Immunohistochemistry-Paraffin: DAGLA Antibody [NBP1-20905] - Staining of paraffin embedded Human Spleen. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: DAGLA Antibody [NBP1-20905]

Immunohistochemistry-Paraffin: DAGLA Antibody [NBP1-20905]

Immunohistochemistry-Paraffin: DAGLA Antibody [NBP1-20905] - Staining of paraffin embedded Human Breast. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: DAGLA Antibody [NBP1-20905]

Immunohistochemistry-Paraffin: DAGLA Antibody [NBP1-20905]

Immunohistochemistry-Paraffin: DAGLA Antibody [NBP1-20905] - Staining of paraffin embedded Human Pancreas. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for DAGLA Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

5 ug/mL

Peptide ELISA

Detection limit 1:128000

Western Blot

0.3 - 1 ug/mL
Application Notes
WB: Approx. 125 kDa band observed in human liver lysates (calculated MW of 115 kDa band according to NP_006124.1). Some minor background is detected and is blocked by the immunizing peptide. We call for caution when used for other assays than WB.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DAGLA

Long Name

Diacylglycerol lipase-alpha

Alternate Names

C11orf11, DAGL-alpha, DGL-alpha, EC 3.1.1.116, KIAA0659, NSDDR

Entrez Gene IDs

747 (Human); 269060 (Mouse); 309207 (Rat)

Gene Symbol

DAGLA

UniProt

NP_006124.1 (Human)

Additional DAGLA Products

Product Documents for DAGLA Antibody

Certificate of Analysis

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Product Specific Notices for DAGLA Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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