DARS Antibody - BSA Free

Immunohistochemistry-Paraffin: DARS Antibody - BSA Free [NBP1-85937]

Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.

Immunohistochemistry-Paraffin: DARS Antibody - BSA Free [NBP1-85937]

Staining of human cerebral cortex, gastrointestinal, kidney and pancreas using Anti-DARS antibody NBP1-85937 (A) shows similar protein distribution across tissues to independent antibody NBP1-86027 (B).

Western Blot: DARS Antibody - BSA Free [NBP1-85937]

Analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-DARS antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Western Blot: DARS Antibody - BSA Free [NBP1-85937] -

DARS expression in the adult human brain. (A,B) Levels of DARS(A) and DARS2(B) mRNA across different brain regions [CX, motor cortex; HC, hippocampus; BS, brainstem (pons); CB, cerebellum; ST, striatum; CC, corpus callosum] determined by qRT-PCR. DARS and DARS2 mRNA were normalized to the housekeeping gene beta -Actin (n = 5). (C) Western-blot detection of DARS in lysates from HEK 293 cells ectopically expressing human DARS including an N-terminal FLAG-HA-tag or a control plasmid. DARS protein was detected with a monoclonal mouse antibody from SantaCruz (m alpha DARS), or a polyclonal rabbit antibody from Novus Biologicals (rb alpha DARS), or an HA antibody. beta -Actin served as internal standard. (D) Representative Western-blot depicting the expression of DARS, beta -Actin, and GAPDH across different brain regions. (E) Levels of DARS protein (detected with m alpha DARS) normalized to beta -Actin (n = 5). Data represent mean +/- SEM (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001; one-way ANOVA). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29615866), licensed under a CC-BY license. Not internally tested by Novus Biologicals.