DDX11 Antibody (3C1) - Azide and BSA Free

Novus Biologicals | Catalog # H00001663-M03

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 3C1

Format

Azide and BSA Free
View all DDX11 Primary Antibodies »

Product Specifications

Immunogen

DDX11 (AAH11264, 1 a.a. ~ 970 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MANETQKVGAIHFPFPFTPYSIQEDFMAELYRVLEAGKIGIFESPTGTGKSLSLICGALSWLRDFEQKKREEEARLLETGTGPLHDEKDESLCLSSSCEGAAGTPRPAGEPAWVTQFVQKKEERDLVDRLKAEQARRKQREERLQQLQHRVQLKYAAKRLRQEEEERENLLRLSREMLETGPEAERLEQLESGEEELVLAEYESDEEKKVASRVDEDEDDLEEEHITKIYYCSRTHSQLAQFVHEVKKSPFGKDVRLVSLGSRQNLCVNEDVKSLGSVQLINDRCVDMQRSRHEKKKGAEEEKPKRRRQEKQAACPFYNHEQMGLLRDEALAEVKDMEQLLALGKEARACPYYGSRLAIPAAQLVVLPYQMLLHAATRQAAGIRLQDQVVIIDEAHNLIDTITGMHSVEVSGSQLCQAHSQLLQYVERYGKRLKAKNLMYLKQILYLLEKFVAVLGGNIKQNPNTQSLSQTGTELKTINDFLFQSQIDNINLFKVQRYCEKSMISRKLFGFTERYGAVFSSREQPKLAGFQQFLQSLQPRTTEALAAPADESQASTLRPASPLMHIQGFLAALTTANQDGRVILSRQGSLSQSTLKFLLLNPAVHFAQVVKECRAVVIAGGTMQPVSDFRQQLLACAGVEAERVVEFSCGHVIPPDNILPLVICSGISNQPLEFTFQKRELPQMMDEVGRILCNLCGVVPGGVVCFFPSYEYLRQVHAHWEKGGLLGRLAARKKIFQEPKSAHQVEQVLLAYSRCIQACGQERGQVTGALLLSVVGGKMSEGINFSDNLGRCVVMVGMPFPNIRSAELQEKMAYLDQTLSPRPGTPREGSGGEPVHEGRQPVHRQGHQAPEGFCQRSAPGPAICPAPCPGQAAGLDPSPCGGQSYLWPRHCCCAEVSPGEVGLFLMGNHATAWRRALPLSCPLETVFVVGVVCGDPVTKVKPRRRVWSPECCQDPGTGVSSRRRKWGNPE

Specificity

DDX11 (3C1)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for DDX11 Antibody (3C1) - Azide and BSA Free

Western Blot: DDX11 Antibody (3C1) [H00001663-M03]

Western Blot: DDX11 Antibody (3C1) [H00001663-M03]

Western Blot: DDX11 Antibody (3C1) [H00001663-M03] - Analysis of DDX11 expression in human liver.
Immunocytochemistry/ Immunofluorescence: DDX11 Antibody (3C1) [H00001663-M03]

Immunocytochemistry/ Immunofluorescence: DDX11 Antibody (3C1) [H00001663-M03]

Immunocytochemistry/Immunofluorescence: DDX11 Antibody (3C1) [H00001663-M03] - Analysis of monoclonal antibody to DDX11 on HeLa cell. Antibody concentration 10 ug/ml
Immunohistochemistry-Paraffin: DDX11 Antibody (3C1) [H00001663-M03]

Immunohistochemistry-Paraffin: DDX11 Antibody (3C1) [H00001663-M03]

Immunohistochemistry-Paraffin: DDX11 Antibody (3C1) [H00001663-M03] - Analysis of monoclonal antibody to DDX11 on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml
Western Blot: DDX11 Antibody (3C1) [H00001663-M03]

Western Blot: DDX11 Antibody (3C1) [H00001663-M03]

Western Blot: DDX11 Antibody (3C1) [H00001663-M03] - Analysis of DDX11 expression in HeLa(Cat # L013V1).
Sandwich ELISA: DDX11 Antibody (3C1) [H00001663-M03] - Detection limit for recombinant GST tagged DDX11 is 3 ng/ml as a capture antibody.

Applications for DDX11 Antibody (3C1) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
It has been used for ELISA and WB.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: DDX11

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an enzyme that possesses both ATPase and DNA helicase activities. This gene is a homolog of the yeast CHL1 gene, and may function to maintain chromosome transmission fidelity and genome stability. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq]

Alternate Names

CHL1CHL1-related helicase gene-1, CHL1-related protein 1, CHLR1MGC9335, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 11, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 11 (CHL1-like helicase homolog, S.cerevisiae), DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 11 (S.cerevisiae CHL1-likehelicase), DEAD/H box protein 11, EC 3.6.1, EC 3.6.1.23, EC 3.6.1.7, EC 3.6.4.13, hCHLR1, Keratinocyte growth factor-regulated gene 2 protein, KRG-2, KRG2CHL1-like helicase homolog, MGC133249, probable ATP-dependent RNA helicase DDX11

Entrez Gene IDs

1663 (Human)

Gene Symbol

DDX11

UniProt

AAH11264 (Human)

Additional DDX11 Products

Product Documents for DDX11 Antibody (3C1) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for DDX11 Antibody (3C1) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for DDX11 Antibody (3C1) - Azide and BSA Free

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Protocols

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FAQs

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