DGCR14 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-84258

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all DGCR14 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ASASSLLLPAASRPPRKREAGEAGAATSKQRVLDEEEYIEGLQTVIQRDFFPDVEKLQAQKEYLEAEENGDLERMRQIAIKFGSALGKMSREPPPPYVTPATFETPEVHAGTGVVGNKPRPRGRGLEDGEAGEEEEKEPLPSLDV

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (86%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DGCR14 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: DGCR14 Antibody [NBP1-84258]

Immunocytochemistry/ Immunofluorescence: DGCR14 Antibody [NBP1-84258]

Immunocytochemistry/Immunofluorescence: DGCR14 Antibody [NBP1-84258] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: DGCR14 Antibody [NBP1-84258]

Immunohistochemistry-Paraffin: DGCR14 Antibody [NBP1-84258]

Immunohistochemistry-Paraffin: DGCR14 Antibody [NBP1-84258] - Staining of human cerebral cortex, colon, liver and testis using Anti-DGCR14 antibody NBP1-84258 (A) shows similar protein distribution across tissues to independent antibody NBP1-84257 (B).
Immunohistochemistry-Paraffin: DGCR14 Antibody [NBP1-84258]

Immunohistochemistry-Paraffin: DGCR14 Antibody [NBP1-84258]

Immunohistochemistry-Paraffin: DGCR14 Antibody [NBP1-84258] - Staining of human colon using Anti-DGCR14 antibody.
Immunohistochemistry-Paraffin: DGCR14 Antibody [NBP1-84258]

Immunohistochemistry-Paraffin: DGCR14 Antibody [NBP1-84258]

Immunohistochemistry-Paraffin: DGCR14 Antibody [NBP1-84258] - Staining of human cerebral cortex using Anti-DGCR14 antibody.
Immunohistochemistry-Paraffin: DGCR14 Antibody [NBP1-84258]

Immunohistochemistry-Paraffin: DGCR14 Antibody [NBP1-84258]

Immunohistochemistry-Paraffin: DGCR14 Antibody [NBP1-84258] - Staining of human testis using Anti-DGCR14 antibody.
DGCR14 Antibody - BSA Free Immunohistochemistry-Paraffin: DGCR14 Antibody - BSA Free [NBP1-84258]

Immunohistochemistry-Paraffin: DGCR14 Antibody - BSA Free [NBP1-84258]

Staining of human liver using Anti-DGCR14 antibody HPA001221.
DGCR14 Antibody - BSA Free Western Blot: DGCR14 Antibody - BSA Free [NBP1-84258]

Western Blot: DGCR14 Antibody - BSA Free [NBP1-84258]

Analysis in control (vector only transfected HEK293T lysate) and DGCR14 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY411609).

Applications for DGCR14 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DGCR14

DGCR14, also known as DiGeorge syndrome critical region 14, is a 52.6 kDa, 476 amino acid protein that exists in the region thought to be responsible for multiple developmental disorders. Current research is studying the involvement of the protein in diseases and disorders such as DiGeorge syndrome, schizophrenia, velocardiofacial syndrome, and faces syndrome. The protein may be involved in mRNA splicing pathways where it interacts with PFDN1, VIM, TTC14, GNB2L1, and FRA10AC1.

Alternate Names

DGCR13, DGS-HDGS-I, DGSIDGSH, DiGeorge syndrome critical region 13, DiGeorge syndrome critical region 14, DiGeorge syndrome critical region gene 13, DiGeorge syndrome critical region gene 14, DiGeorge syndrome gene H, DiGeorge syndrome gene I, DiGeorge syndrome protein H, ES2DiGeorge syndrome critical region gene DGSI, Es2el, Protein DGCR13, protein DGCR14, Protein ES2

Gene Symbol

ESS2

Additional DGCR14 Products

Product Documents for DGCR14 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for DGCR14 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for DGCR14 Antibody - BSA Free

There are currently no reviews for this product. Be the first to review DGCR14 Antibody - BSA Free and earn rewards!

Have you used DGCR14 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies