DHX8 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-13923

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all DHX8 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: PDGSLSQAAMMQSALAKERRELKQAQREAEMDSIPMGLNKHWVDPLPDAEGRQIAANMRGIGMMPNDIPEWKKHAFGGNKASYGKKTQMSILEQRESLP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DHX8 Antibody - BSA Free

Western Blot: DHX8 Antibody [NBP2-13923]

Western Blot: DHX8 Antibody [NBP2-13923]

Western Blot: DHX8 Antibody [NBP2-13923] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.
Immunocytochemistry/ Immunofluorescence: DHX8 Antibody [NBP2-13923]

Immunocytochemistry/ Immunofluorescence: DHX8 Antibody [NBP2-13923]

Immunocytochemistry/Immunofluorescence: DHX8 Antibody [NBP2-13923] - Staining of human cell line HEK 293 shows positivity in nucleus but not nucleoli. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: DHX8 Antibody [NBP2-13923]

Immunohistochemistry-Paraffin: DHX8 Antibody [NBP2-13923]

Immunohistochemistry-Paraffin: DHX8 Antibody [NBP2-13923] - Staining of human placenta shows moderate nuclear positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: DHX8 Antibody [NBP2-13923]

Immunohistochemistry-Paraffin: DHX8 Antibody [NBP2-13923]

Immunohistochemistry-Paraffin: DHX8 Antibody [NBP2-13923] - Staining of human cerebellum shows strong cytoplasmic and nucleolar positivity in Purkinje cells.
Immunohistochemistry-Paraffin: DHX8 Antibody [NBP2-13923]

Immunohistochemistry-Paraffin: DHX8 Antibody [NBP2-13923]

Immunohistochemistry-Paraffin: DHX8 Antibody [NBP2-13923] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: DHX8 Antibody [NBP2-13923]

Immunohistochemistry-Paraffin: DHX8 Antibody [NBP2-13923]

Immunohistochemistry-Paraffin: DHX8 Antibody [NBP2-13923] - Staining of human skin shows moderate nuclear positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: DHX8 Antibody [NBP2-13923]

Immunohistochemistry-Paraffin: DHX8 Antibody [NBP2-13923]

Immunohistochemistry-Paraffin: DHX8 Antibody [NBP2-13923] - Staining of human liver shows no positivity in hepatocytes as expected.

Applications for DHX8 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF Fixation/Permeabilization: PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DHX8

DHX8 is a member of the DEAD box protein family, which is characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD). The DEAD or DEAH motif is characteristic of a family of putative ATP-dependent RNA helicases. DHX8 has a role in nuclear export of spliced mRNA by releasing the RNA from the spliceosome.

Alternate Names

DDX8ATP-dependent RNA helicase DHX8, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 8 (RNA helicase), DEAH (Asp-Glu-Ala-His) box polypeptide 8, DEAH box protein 8, DEAH-box protein 8, EC 3.6.1, EC 3.6.4.13, HRH1RNA helicase HRH1, PRP22, PRPF22

Gene Symbol

DHX8

Additional DHX8 Products

Product Documents for DHX8 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for DHX8 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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