DKFZP434B168 Antibody - BSA Free
Novus Biologicals | Catalog # NB100-465
![Western Blot: DKFZP434B168 Antibody [NB100-465]](https://resources.rndsystems.com/images/products/DKFZP434B168-Antibody-Western-Blot-NB100-465-img0008.jpg "Western Blot: DKFZP434B168 Antibody [NB100-465]")
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
The immunogen this antibody was made to, maps to a region between residues 850-900 of human DKFZP434B168 using the TrEMBL entry Q9NVH2 (GeneID 25896).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for DKFZP434B168 Antibody - BSA Free
Western Blot: DKFZP434B168 Antibody [NB100-465]
Western Blot: DKFZP434B168 Antibody [NB100-465] - Detection of Human INT7 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-INT7 antibody NB100-465 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.![Immunohistochemistry-Paraffin: DKFZP434B168 Antibody [NB100-465]](https://resources.rndsystems.com/images/products/DKFZP434B168-Antibody-Immunohistochemistry-Paraffin-NB100-465-img0003.jpg "Immunohistochemistry-Paraffin: DKFZP434B168 Antibody [NB100-465]")
Immunohistochemistry-Paraffin: DKFZP434B168 Antibody [NB100-465]
Immunohistochemistry-Paraffin: DKFZP434B168 Antibody [NB100-465] - Human breast carcinoma. Antibody used at a dilution of 1:1000 (1ug/ml).![Western Blot: DKFZP434B168 Antibody [NB100-465]](https://resources.rndsystems.com/images/products/DKFZP434B168-Antibody-Western-Blot-NB100-465-img0004.jpg "Western Blot: DKFZP434B168 Antibody [NB100-465]")
Western Blot: DKFZP434B168 Antibody [NB100-465]
Western Blot: DKFZP434B168 Antibody [NB100-465] - Nuclear extract (NE) from HeLa cells. Antibody used at 0.1 ug/ml.![Immunohistochemistry-Paraffin: DKFZP434B168 Antibody [NB100-465]](https://resources.rndsystems.com/images/products/DKFZP434B168-Antibody-Immunohistochemistry-Paraffin-NB100-465-img0001.jpg "Immunohistochemistry-Paraffin: DKFZP434B168 Antibody [NB100-465]")
Immunohistochemistry-Paraffin: DKFZP434B168 Antibody [NB100-465]
Immunohistochemistry-Paraffin: DKFZP434B168 Antibody [NB100-465] - FFPE section of human breast carcinoma Affinity purified rabbit anti-INT7 used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory KitApplications for DKFZP434B168 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:500 - 1:2000
Western Blot
1:10000-1:25000
Application Notes
Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: DKFZP434B168
Alternate Names
C1orf73, chromosome 1 open reading frame 73, DKFZP434B168, Int7, integrator complex subunit 7
Entrez Gene IDs
25896 (Human)
Gene Symbol
INTS7
UniProt
Additional DKFZP434B168 Products
Product Documents for DKFZP434B168 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for DKFZP434B168 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for DKFZP434B168 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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