![Western Blot: DLL1 Antibody [NB600-628]](https://resources.rndsystems.com/images/products/DLL1-Antibody-Western-Blot-NB600-628-img0002.jpg "Western Blot: DLL1 Antibody [NB600-628]")
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Key Product Details
Species Reactivity
Validated:
Mouse
Predicted:
Human (96%), Rat (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Western Blot, ELISA
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This affinity purified DLL1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 150-175 of Mouse DLL1. (Uniprot: Q61483)
Reactivity Notes
Activity against DLL11 from other species has not been determined but is expected based on sequence homology. Expected to cross-react with rat, human and chicken.
Specificity
The antibody detects DLL1 from mouse. Activity against Delta-1 from other species has not been determined but is expected based on sequence homology. Expected to cross-react with rat, human and chicken.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody was affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for DLL1 Antibody - BSA Free
Western Blot: DLL1 Antibody [NB600-628]
Western Blot: DLL1 Antibody [NB600-628]![Immunohistochemistry: DLL1 Antibody [NB600-628]](https://resources.rndsystems.com/images/products/DLL1-Antibody-Immunohistochemistry-NB600-628-img0008.jpg "Immunohistochemistry: DLL1 Antibody [NB600-628]")
Immunohistochemistry: DLL1 Antibody [NB600-628]
Immunohistochemistry: DLL1 Antibody [NB600-628] - Immunohistochemistry of DLL1 antibodyTissue: Mouse Embryonic Kidney. Fixation: FFPE buffered formalin 10% conc.Ag Retrieval: Heat, Citrate pH 6.2. Pressure Cooker (left, right), EDTA pH 9.5 Pressure Cooker (middle).Primary antibody: 2ug/ml 1.5 hour @ room temp.Secondary Ab: MACH 1 HRP POLYMER at 1:50 for 45" RT.![Immunohistochemistry: DLL1 Antibody [NB600-628]](https://resources.rndsystems.com/images/products/DLL1-Antibody-Immunohistochemistry-NB600-628-img0007.jpg "Immunohistochemistry: DLL1 Antibody [NB600-628]")
Immunohistochemistry: DLL1 Antibody [NB600-628]
Immunohistochemistry: DLL1 Antibody [NB600-628] - Immunohistochemistry of DLL1 antibody. Tissue: Mouse embryonic kidney.Fixation: formalin fixed paraffin embedded 10% conc.Antigen retrieval: pH 9.5. Primary antibody: 2ug/ml for 1.5 hour @ room Temp.Secondary antibody: Peroxidase secondary reagent for 45 min at RT.Localization: as shown.Staining: 3.3’-diaminobenzidine tetrahydrochloride was used as the chromogen. Nuclei were counterstained purple with hematoxylin.![ELISA: DLL1 Antibody [NB600-628]](https://resources.rndsystems.com/images/products/DLL1-Antibody-ELISA-NB600-628-img0009.jpg "ELISA: DLL1 Antibody [NB600-628]")
ELISA: DLL1 Antibody [NB600-628]
ELISA: DLL1 Antibody [NB600-628] - ELISA results of purified DLL1 antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1ug of conjugate. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB ELISA Peroxidase Substrate.
DLL1 Antibody
Immunohistochemistry of Rabbit Anti-Delta1 antibody. Tissue: Mouse embryonic kidney. Fixation: formalin fixed paraffin embedded 10% conc. Antigen retrieval: pH 9.5. Primary antibody: 2ug/ml for 1.5 hour @ room Temp. Secondary antibody: Peroxidase secondary reagent for 45 min at RT. Localization: as shown. Staining: 3.3'-diaminobenzidine tetrahydrochloride was used as the chromogen. Nuclei were counterstained purple with hematoxylin.
DLL1 Antibody
Immunohistochemistry of Rabbit anti-mouse DELTA1 Antibody in Mouse Embryonic Kidney Tissue: Mouse Embryonic Kidney. Fixation: FFPE buffered formalin 10% conc. Ag Retrieval: Heat, Citrate pH 6.2. Pressure Cooker (left, right), EDTA pH 9.5 Pressure Cooker (middle). Primary antibody: 2ug/ml 1.5 hour @ room temp. Secondary Ab: MACH 1 HRP POLYMER at 1:50 for 45" RT.Applications for DLL1 Antibody - BSA Free
Application
Recommended Usage
ELISA
1:3000-1:12000
Immunohistochemistry
2ug/ml
Western Blot
1:100-1:1000
Application Notes
This product has been tested for use in ELISA against the immunizing peptide, western blot, and in IHC. Reactivity in other immunoassays is unknown.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Format
BSA Free
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: DLL1
Additional DLL1 Products
Product Documents for DLL1 Antibody - BSA Free
Product Specific Notices for DLL1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
