DNA Polymerase iota Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-55989

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all DNA Polymerase iota Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: PKDKETNRDFLPSGRIESTRTRESPLDTTNFSKEKDINEFPLCSLPEGVDQEVFKQLPVDIQEEILSGKSREKFQGKGSVSCPL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DNA Polymerase iota Antibody - BSA Free

Immunohistochemistry-Paraffin: DNA Polymerase iota Antibody [NBP2-55989]

Immunohistochemistry-Paraffin: DNA Polymerase iota Antibody [NBP2-55989]

Immunohistochemistry-Paraffin: DNA Polymerase iota Antibody [NBP2-55989] - Staining of human testis shows high expression.
Immunohistochemistry-Paraffin: DNA Polymerase iota Antibody [NBP2-55989]

Immunohistochemistry-Paraffin: DNA Polymerase iota Antibody [NBP2-55989]

Immunohistochemistry-Paraffin: DNA Polymerase iota Antibody [NBP2-55989] - Staining of human skeletal muscle shows low expression as expected.
Immunohistochemistry-Paraffin: DNA Polymerase iota Antibody [NBP2-55989]

Immunohistochemistry-Paraffin: DNA Polymerase iota Antibody [NBP2-55989]

Immunohistochemistry-Paraffin: DNA Polymerase iota Antibody [NBP2-55989] - Staining in human testis and skeletal muscle tissues using anti-POLI antibody. Corresponding POLI RNA-seq data are presented for the same tissues.

Applications for DNA Polymerase iota Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DNA Polymerase iota

DNA polymerase Iota (POLI), also known as Rad30B is a novel human polymerase, unlike any others known. It is an extremely low-fidelity enzyme that is highly error-prone when replicating undamaged and damaged DNA in vitro. To date there is, however, no human disease that can be attributed to overexpression or defects in DNA Pol I.

DNA Pol I antibodies are useful for DNA replication and repair studies and in research on mitosis and cell replication.

Alternate Names

DNA polymerase iota, EC 2.7.7.7, eta2, polymerase (DNA directed) iota, RAD30 homolog B, RAD30Bpolymerase (DNA-directed), iota, RAD3OB

Gene Symbol

POLI

Additional DNA Polymerase iota Products

Product Documents for DNA Polymerase iota Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for DNA Polymerase iota Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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