DNAI1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-84466

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all DNAI1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: APHKQPHKQSISIGRGTRKRDEDSGTEVGEGTDEWAQSKATVRPPDQLELTDAELKEEFTRILTANNPHAPQNIVRYSFKEGTYKPIGFVNQLAVHYTQV

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (80%). Reactivity reported in scientific literature (PMID: 24298994)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DNAI1 Antibody - BSA Free

Immunohistochemistry-Paraffin: DNAI1 Antibody [NBP1-84466]

Immunohistochemistry-Paraffin: DNAI1 Antibody [NBP1-84466]

Immunohistochemistry-Paraffin: DNAI1 Antibody [NBP1-84466] - Staining in human testis and prostate tissues using NBP1-84466 antibody. Corresponding DNAI1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: DNAI1 Antibody [NBP1-84466]

Immunohistochemistry-Paraffin: DNAI1 Antibody [NBP1-84466]

Immunohistochemistry-Paraffin: DNAI1 Antibody [NBP1-84466] - Staining of human fallopian tube, placenta, prostate and testis using Anti-DNAI1 antibody NBP1-84466 (A) shows similar protein distribution across tissues to independent antibody NBP1-84465 (B).
Western Blot: DNAI1 Antibody [NBP1-84466]

Western Blot: DNAI1 Antibody [NBP1-84466]

Western Blot: DNAI1 Antibody [NBP1-84466] - Analysis in control (vector only transfected HEK293T lysate) and dNAI1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: DNAI1 Antibody [NBP1-84466]

Immunohistochemistry-Paraffin: DNAI1 Antibody [NBP1-84466]

Immunohistochemistry-Paraffin: DNAI1 Antibody [NBP1-84466] - Staining of human Fallopian tube shows strong positivity in cilia in glandular cells.
Immunohistochemistry-Paraffin: DNAI1 Antibody [NBP1-84466]

Immunohistochemistry-Paraffin: DNAI1 Antibody [NBP1-84466]

Immunohistochemistry-Paraffin: DNAI1 Antibody [NBP1-84466] - Staining of human placenta shows no positivity in trophoblastic cells as expected.
Immunohistochemistry-Paraffin: DNAI1 Antibody [NBP1-84466]

Immunohistochemistry-Paraffin: DNAI1 Antibody [NBP1-84466]

Immunohistochemistry-Paraffin: DNAI1 Antibody [NBP1-84466] - Staining of human prostate shows no positivity in glandular cells as expected.
Immunohistochemistry-Paraffin: DNAI1 Antibody [NBP1-84466]

Immunohistochemistry-Paraffin: DNAI1 Antibody [NBP1-84466]

Immunohistochemistry-Paraffin: DNAI1 Antibody [NBP1-84466] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.

Applications for DNAI1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
WB reported in scientific literature (PMID: 25700162). For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DNAI1

The inner- and outer-arm dyneins, which bridge between the doublet microtubules in axonemes, are the force-generatingproteins responsible for the sliding movement in axonemes. The intermediate and light chains, thought to form the baseof the dynein arm, help mediate attachment and may also participate in regulating dynein activity. This gene encodesan intermediate chain dynein, belonging to the large family of motor proteins. Mutations in this gene result inabnormal ciliary ultrastructure and function associated with primary ciliary dyskinesia (PCD) and Kartagener syndrome.(provided by RefSeq)

Alternate Names

Axonemal dynein intermediate chain 1, CILD1MGC26204, dynein intermediate chain DNAI1, dynein, axonemal, intermediate chain 1, dynein, axonemal, intermediate polypeptide 1, ICS, ICS1, immotile cilia syndrome 1, PCDdynein intermediate chain 1, axonemal

Gene Symbol

DNAI1

Additional DNAI1 Products

Product Documents for DNAI1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for DNAI1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for DNAI1 Antibody - BSA Free

Customer Reviews for DNAI1 Antibody - BSA Free

There are currently no reviews for this product. Be the first to review DNAI1 Antibody - BSA Free and earn rewards!

Have you used DNAI1 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies