DOK5 Antibody

Novus Biologicals | Catalog # NB100-1463

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all DOK5 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence CLHRTETFPAYRSEH corresponding to C-Terminus according to NP_060901.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for DOK5 Antibody

Western Blot: DOK5 Antibody [NB100-1463]

Western Blot: DOK5 Antibody [NB100-1463]

Western Blot: DOK5 Antibody [NB100-1463] - Analysis of DOK5 in Human Kidney lysate (35 ug protein in RIPA buffer) using this antibody at 0.1 ug/ml.
Immunohistochemistry-Paraffin: DOK5 Antibody [NB100-1463]

Immunohistochemistry-Paraffin: DOK5 Antibody [NB100-1463]

Immunohistochemistry-Paraffin: DOK5 Antibody [NB100-1463] - IHC staining of paraffin embedded Human Tonsil using this antibody at 1 ug/ml. Microwaved antigen retrieval with citrate buffer pH6, HRP-staining.
Western Blot: DOK5 Antibody [NB100-1463]

Western Blot: DOK5 Antibody [NB100-1463]

Western Blot: DOK5 Antibody [NB100-1463] - HEK293 overexpressing DOK5 and probed with (mock transfection in first lane).

Applications for DOK5 Antibody

Application
Recommended Usage

Immunohistochemistry

1 - 2 ug/ml

Immunohistochemistry-Paraffin

1 - 2 ug/ml

Peptide ELISA

Detection limit 1:16000

Western Blot

1 - 3 ug/ml
Application Notes
WB: In transfected HEK293 transiently expressing DOK5 a band of approx. 38 kDa is observed. This band is not observed in the non-transfected HEK293. The calculated molecular size is 35.5 kDa band according to NP_060901.2. IHC-P: Tonsil stains scattered cells in the interfollicular area compatible with mast cells.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DOK5

DOK5 is a member of the DOK family of membrane proteins, which are adapter proteins involved in signal transduction. The encoded protein interacts with phosphorylated receptor tyrosine kinases to mediate neurite outgrowth and activation of the MAP kinase pathway. In contrast to other DOK family proteins, this protein does not interact with RASGAP. Two transcript variants encoding two different isoforms have been found for this gene.

Alternate Names

C20orf180, chromosome 20 open reading frame 180, dJ805C22.1, docking protein 5, Downstream of tyrosine kinase 5, Insulin receptor substrate 6, IRS6, IRS-6, MGC16926

Entrez Gene IDs

55816 (Human); 76829 (Mouse)

Gene Symbol

DOK5

UniProt

NP_060901 (Human)

Additional DOK5 Products

Product Documents for DOK5 Antibody

Certificate of Analysis

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Product Specific Notices for DOK5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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