DPM1 Antibody

Novus Biologicals | Catalog # NBP1-05034

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all DPM1 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence RELEVRSPRQNKYS-C corresponding to N-Terminus according to NP_003850.1.

Epitope

N Terminus

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for DPM1 Antibody

Western Blot: DPM1 Antibody [NBP1-05034]

Western Blot: DPM1 Antibody [NBP1-05034]

Western Blot: DPM1 Antibody [NBP1-05034] - (0.1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: DPM1 Antibody [NBP1-05034]

Immunohistochemistry-Paraffin: DPM1 Antibody [NBP1-05034]

Immunohistochemistry-Paraffin: DPM1 Antibody [NBP1-05034] - (3.75ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for DPM1 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

3.75 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

0.1-0.3 ug/ml
Application Notes
Western blot: Approx. 28kDa band observed in Human Liver lysates (calculated MW of 29.6kDa according to NP_003850.1). Primary incubation was 1 hour.
IHC: Paraffin embedded Human Tonsil.).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DPM1

Dolichol-phosphate mannose (Dol-P-Man) serves as a donor of mannosyl residues on the lumenal side of the endoplasmic reticulum (ER). Lack of Dol-P-Man results in defective surface expression of GPI-anchored proteins. Dol-P-Man is synthesized from GDP-mannose and dolichol-phosphate on the cytosolic side of the ER by the enzyme dolichyl-phosphate mannosyltransferase. Human DPM1 lacks a carboxy-terminal transmembrane domain and signal sequence and is regulated by DPM2. [provided by RefSeq]

Alternate Names

CDGIEEC 2.4.1.83, dolichol monophosphate mannose synthase, dolichol-phosphate mannosyltransferase, Dolichyl-phosphate beta-D-mannosyltransferase, dolichyl-phosphate mannosyltransferase polypeptide 1, catalytic subunit, DPM synthase, Mannose-P-dolichol synthase, MPD synthase, MPDSDolichol-phosphate mannose synthase

Entrez Gene IDs

8813 (Human)

Gene Symbol

DPM1

UniProt

NP_003850.1 (Human)

Additional DPM1 Products

Product Documents for DPM1 Antibody

Certificate of Analysis

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Product Specific Notices for DPM1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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