DR1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-48979

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all DR1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: MASSSGNDDDLTIPRAAINKMIKETLPNVRVANDARELVVNCCTEFIHLISSEANEICNKSEKKTISPEHVIQALESLGFG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DR1 Antibody - BSA Free

Western Blot: DR1 Antibody [NBP2-48979]

Western Blot: DR1 Antibody [NBP2-48979]

Western Blot: DR1 Antibody [NBP2-48979] - Analysis in control (vector only transfected HEK293T lysate) and DR1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: DR1 Antibody [NBP2-48979]

Immunohistochemistry-Paraffin: DR1 Antibody [NBP2-48979]

Immunohistochemistry-Paraffin: DR1 Antibody [NBP2-48979] - Staining of human gallbladder.
Immunohistochemistry-Paraffin: DR1 Antibody [NBP2-48979]

Immunohistochemistry-Paraffin: DR1 Antibody [NBP2-48979]

Immunohistochemistry-Paraffin: DR1 Antibody [NBP2-48979] - Staining of human liver shows low expression as expected.
Immunohistochemistry-Paraffin: DR1 Antibody [NBP2-48979]

Immunohistochemistry-Paraffin: DR1 Antibody [NBP2-48979]

Immunohistochemistry-Paraffin: DR1 Antibody [NBP2-48979] - Staining of human testis shows high expression.
Immunohistochemistry-Paraffin: DR1 Antibody [NBP2-48979]

Immunohistochemistry-Paraffin: DR1 Antibody [NBP2-48979]

Immunohistochemistry-Paraffin: DR1 Antibody [NBP2-48979] - Staining in human testis and liver tissues using anti-DR1 antibody. Corresponding DR1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: DR1 Antibody [NBP2-48979]

Immunohistochemistry-Paraffin: DR1 Antibody [NBP2-48979]

Immunohistochemistry-Paraffin: DR1 Antibody [NBP2-48979] - Staining of human cerebral cortex, gallbladder, liver and testis using Anti-DR1 antibody NBP2-48979 (A) shows similar protein distribution across tissues to independent antibody NBP2-47480 (B).
Immunohistochemistry-Paraffin: DR1 Antibody [NBP2-48979]

Immunohistochemistry-Paraffin: DR1 Antibody [NBP2-48979]

Immunohistochemistry-Paraffin: DR1 Antibody [NBP2-48979] - Staining of human cerebral cortex.

Applications for DR1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DR1

DR1 encodes a TBP- (TATA box-binding protein) associated phosphoprotein that represses both basal and activated levels of transcription. The encoded protein is phosphorylated in vivo and this phosphorylation affects its interaction with TBP. This protein contains a histone fold motif at the amino terminus, a TBP-binding domain, and a glutamine- and alanine-rich region. The binding of DR1 repressor complexes to TBP-promoter complexes may establish a mechanism in which an altered DNA conformation, together with the formation of higher order complexes, inhibits the assembly of the preinitiation complex and controls the rate of RNA polymerase II transcription. [provided by RefSeq]

Alternate Names

Down-regulator of transcription 1, down-regulator of transcription 1, TBP-binding (negative cofactor 2), NC2, NC2-BETA, Negative cofactor 2-beta, protein Dr1, TATA-binding protein-associated phosphoprotein

Gene Symbol

DR1

Additional DR1 Products

Product Documents for DR1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for DR1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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